2f1d
From Proteopedia
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|PDB= 2f1d |SIZE=350|CAPTION= <scene name='initialview01'>2f1d</scene>, resolution 3.00Å | |PDB= 2f1d |SIZE=350|CAPTION= <scene name='initialview01'>2f1d</scene>, resolution 3.00Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene> | + | |LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Imidazoleglycerol-phosphate_dehydratase Imidazoleglycerol-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.19 4.2.1.19] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Imidazoleglycerol-phosphate_dehydratase Imidazoleglycerol-phosphate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.19 4.2.1.19] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f1d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f1d OCA], [http://www.ebi.ac.uk/pdbsum/2f1d PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f1d RCSB]</span> | ||
}} | }} | ||
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[[Category: Rice, D W.]] | [[Category: Rice, D W.]] | ||
[[Category: Sedelnikova, S E.]] | [[Category: Sedelnikova, S E.]] | ||
| - | [[Category: MN]] | ||
| - | [[Category: SO4]] | ||
[[Category: herbicide]] | [[Category: herbicide]] | ||
[[Category: histidine biosynthesis]] | [[Category: histidine biosynthesis]] | ||
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[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:56:35 2008'' |
Revision as of 23:56, 30 March 2008
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| , resolution 3.00Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Activity: | Imidazoleglycerol-phosphate dehydratase, with EC number 4.2.1.19 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
X-Ray Structure of imidazoleglycerol-phosphate dehydratase
Overview
The structure of A. thaliana imidazoleglycerol-phosphate dehydratase, an enzyme of histidine biosynthesis and a target for the triazole phosphonate herbicides, has been determined to 3.0 A resolution. The structure is composed of 24 identical subunits arranged in 432 symmetry and shows how the formation of a novel dimanganese cluster is crucial to the assembly of the active 24-mer from an inactive trimeric precursor and to the formation of the active site of the enzyme. Molecular modeling suggests that the substrate is bound to the manganese cluster as an imidazolate moiety that subsequently collapses to yield a diazafulvene intermediate. The mode of imidazolate recognition exploits pseudosymmetry at the active site arising from a combination of the assembly of the particle and the pseudosymmetry present in each subunit as a result of gene duplication. This provides an intriguing example of the role of evolution in the design of Nature's catalysts.
About this Structure
2F1D is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Structure and mechanism of imidazoleglycerol-phosphate dehydratase., Glynn SE, Baker PJ, Sedelnikova SE, Davies CL, Eadsforth TC, Levy CW, Rodgers HF, Blackburn GM, Hawkes TR, Viner R, Rice DW, Structure. 2005 Dec;13(12):1809-17. PMID:16338409
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