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2vcp

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Revision as of 06:58, 28 February 2018

Crystal structure of N-Wasp VC domain in complex with skeletal actin

Structural highlights

2vcp is a 4 chain structure with sequence from Human and Oryctolagus cuniculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
NonStd Res:
Related:	1lcu, 2a42, 2aso, 1ijj, 1wua, 1o1a, 1m8q, 1o18, 1uy5, 1rfq, 2d1k, 1ma9, 1rdw, 1o1b, 1o1d, 2a40, 2a5x, 1qz5, 1nwk, 1j6z, 1sqk, 1atn, 1s22, 1t44, 2ff3, 1eqy, 1mvw, 2ff6, 2fxu, 1o1f, 2asp, 1kxp, 1rgi, 2v39, 1o19, 1y64, 1alm, 1o1e, 1p8z, 1esv, 1o1c, 1h1v, 1o1g, 2a3z, 1lot, 2asm, 2a41, 1qz6
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ACTS_RABIT] Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [WASL_HUMAN] Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Wiskott-Aldrich Syndrome Proteins (WASP) are a family of proteins that all catalyze actin filament branching with Arp2/3 complex in a variety of actin-based motile processes. The constitutively active C-terminal domain, called VCA, harbors one or more WASP Homology 2 (WH2) domains that bind G-actin, while the CA extension binds Arp2/3 complex. The VCA-actin-Arp2/3 entity associates with a mother filament to form a branched junction from which a daughter filament is initiated. The number and function of WH2-bound actin(s) in the branching process is not known, and the stoichiometry of VCA-actin-Arp2/3 complex is debated. We have expressed the tandem WH2 repeats of N-WASP, either alone (V) or associated with the C (VC) and CA (VCA) extensions. We analyze the structure of actin in complex with V, VC and VCA using protein crystallography, hydrodynamic and spectrofluorimetric methods. The partial crystal structure of VC :actin 1 :1 complex shows two actins in the asymmetric unit with extensive actin-actin contacts. In solution, each of the 2 WH2 domains in V, VC and VCA binds G-actin in 1 :2 complexes that participate in barbed end assembly. V, VC and VCA enhance barbed end depolymerization like profilin, but neither nucleate nor sever filaments, in contrast with other WH2 repeats. VCA binds Arp2/3 complex in a 1 :1 complex even in the presence of a large excess of VCA. VCA-Arp2/3 binds one actin in a Latrunculin A-sensitive fashion, in a 1 :1 :1 complex, indicating that binding of the second actin to VCA is weakened in the ternary complex.

Interactions of isolated C-terminal fragments of Neural Wiskott-Aldrich Syndrome Protein (N-WASP) with actin and Arp2/3 complex.,Gaucher JF, Mauge C, Didry D, Guichard B, Renault L, Carlier MF J Biol Chem. 2012 Jul 30. PMID:22847007^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Vingadassalom D, Kazlauskas A, Skehan B, Cheng HC, Magoun L, Robbins D, Rosen MK, Saksela K, Leong JM. Insulin receptor tyrosine kinase substrate links the E. coli O157:H7 actin assembly effectors Tir and EspF(U) during pedestal formation. Proc Natl Acad Sci U S A. 2009 Apr 21;106(16):6754-9. doi:, 10.1073/pnas.0809131106. Epub 2009 Apr 6. PMID:19366662 doi:10.1073/pnas.0809131106
↑ Gaucher JF, Mauge C, Didry D, Guichard B, Renault L, Carlier MF. Interactions of isolated C-terminal fragments of Neural Wiskott-Aldrich Syndrome Protein (N-WASP) with actin and Arp2/3 complex. J Biol Chem. 2012 Jul 30. PMID:22847007 doi:10.1074/jbc.M112.394361

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2vcp"

@@ Line 1: / Line 1: @@
 ==Crystal structure of N-Wasp VC domain in complex with skeletal actin==
 <StructureSection load='2vcp' size='340' side='right' caption='[[2vcp]], [[Resolution|resolution]] 3.20&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1lcu|1lcu]], [[2a42|2a42]], [[2aso|2aso]], [[1ijj|1ijj]], [[1wua|1wua]], [[1o1a|1o1a]], [[1m8q|1m8q]], [[1o18|1o18]], [[1uy5|1uy5]], [[1rfq|1rfq]], [[2d1k|2d1k]], [[1ma9|1ma9]], [[1rdw|1rdw]], [[1o1b|1o1b]], [[1o1d|1o1d]], [[2a40|2a40]], [[2a5x|2a5x]], [[1qz5|1qz5]], [[1nwk|1nwk]], [[1j6z|1j6z]], [[1sqk|1sqk]], [[1atn|1atn]], [[1s22|1s22]], [[1t44|1t44]], [[2ff3|2ff3]], [[1eqy|1eqy]], [[1mvw|1mvw]], [[2ff6|2ff6]], [[2fxu|2fxu]], [[1o1f|1o1f]], [[2asp|2asp]], [[1kxp|1kxp]], [[1rgi|1rgi]], [[2v39|2v39]], [[1o19|1o19]], [[1y64|1y64]], [[1alm|1alm]], [[1o1e|1o1e]], [[1p8z|1p8z]], [[1esv|1esv]], [[1o1c|1o1c]], [[1h1v|1h1v]], [[1o1g|1o1g]], [[2a3z|2a3z]], [[1lot|1lot]], [[2asm|2asm]], [[2a41|2a41]], [[1qz6|1qz6]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vcp OCA], [http://pdbe.org/2vcp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vcp RCSB], [http://www.ebi.ac.uk/pdbsum/2vcp PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2vcp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2vcp OCA], [http://pdbe.org/2vcp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2vcp RCSB], [http://www.ebi.ac.uk/pdbsum/2vcp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2vcp ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vc/2vcp_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/vc/2vcp_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 29: / Line 30: @@
 </div>
 <div class="pdbe-citations 2vcp" style="background-color:#fffaf0;"></div>
-==See Also==
-*[[Actin|Actin]]
-*[[Wiskott-Aldrich syndrome protein|Wiskott-Aldrich syndrome protein]]
 == References ==
 <references/>

2vcp

From Proteopedia

Revision as of 06:58, 28 February 2018

Crystal structure of N-Wasp VC domain in complex with skeletal actin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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