2f56
From Proteopedia
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|PDB= 2f56 |SIZE=350|CAPTION= <scene name='initialview01'>2f56</scene>, resolution 1.955Å | |PDB= 2f56 |SIZE=350|CAPTION= <scene name='initialview01'>2f56</scene>, resolution 1.955Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=URE:UREA'>URE</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1a2p|1A2P]], [[2f4y|2F4Y]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f56 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f56 OCA], [http://www.ebi.ac.uk/pdbsum/2f56 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2f56 RCSB]</span> | ||
}} | }} | ||
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[[Category: Prange, T.]] | [[Category: Prange, T.]] | ||
[[Category: Salem, M.]] | [[Category: Salem, M.]] | ||
| - | [[Category: URE]] | ||
| - | [[Category: ZN]] | ||
[[Category: barnase]] | [[Category: barnase]] | ||
[[Category: bromoethanol]] | [[Category: bromoethanol]] | ||
| Line 35: | Line 36: | ||
[[Category: urea]] | [[Category: urea]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:58:02 2008'' |
Revision as of 23:58, 30 March 2008
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| , resolution 1.955Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1A2P, 2F4Y
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Barnase cross-linked with glutaraldehyde soaked in 6M urea
Overview
Structural data about the early step of protein denaturation were obtained from cross-linked crystals for two small proteins: barnase and lysozyme. Several denaturant agents like urea, bromoethanol or thiourea were used at increasing concentrations up to a limit leading to crystal disruption (>or=2 to 6 M). Before the complete destruction of the crystal order started, specific binding sites were observed at the protein surfaces, an indication that the preliminary step of denaturation is the disproportion of intermolecular polar bonds to the benefit of the agent "parasiting" the surface. The analysis of the thermal factors first agree with a stabilization effect at low or moderate concentration of denaturants rapidly followed by a destabilization at specific weak points when the number of sites increase (overflooding effect).
About this Structure
2F56 is a Single protein structure of sequence from Bacillus amyloliquefaciens. Full crystallographic information is available from OCA.
Reference
On the edge of the denaturation process: application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations., Salem M, Mauguen Y, Prange T, Biochim Biophys Acta. 2006 May;1764(5):903-12. Epub 2006 Mar 20. PMID:16600702
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