1qwq

From Proteopedia

(Difference between revisions)

Revision as of 08:03, 28 February 2018

Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease

Structural highlights

1qwq is a 1 chain structure with sequence from Bovin. This structure supersedes the now removed PDB entry 1o0g. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1bsr
Gene:	SRN (BOVIN)
Activity:	Pancreatic ribonuclease, with EC number 3.1.27.5
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RNS_BOVIN] This enzyme hydrolyzes both single- and double-stranded RNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Bovine seminal ribonuclease (BS-RNase), the only dimeric protein among the pancreatic-like ribonucleases, is endowed with special structural features and with biological functions beyond enzymatic activity. In solution, the protein exists as an equilibrium mixture of two forms, with or without exchange (or swapping) of the N-terminal arms. After selective reduction and alkylation of the two intrachain disulfide bridges, the dimeric protein can be transformed into a monomeric derivative that has a ribonuclease activity higher than that of the parent dimeric protein but is devoid of the special biological functions. A detailed investigation of the structural features of this protein in solution, in comparison with those of other monomeric ribonucleases, may help unveil the structural details which induce swapping of the N-terminal arms of BS-RNase. The solution structure of the recombinant monomeric form of BS-RNase, as determined by 3D heteronuclear NMR, shows close similarity with that of bovine pancreatic ribonuclease (RNase A) in all regions characterized by regular elements of secondary structure. However, significant differences are present in the flexible regions, which could account for the different behavior of the two proteins. To characterize in detail these regions, we have measured H/D exchange rate constants, temperature coefficients and heteronuclear NOEs of backbone amides for both RNase A and monomeric BS-RNase. The results indicate a large difference in the backbone flexibility of the hinge peptide segment 16-22 of the two proteins, which could provide the molecular basis to explain the ability of BS-RNase subunits to swap their N-terminal arms.

The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases.,Avitabile F, Alfano C, Spadaccini R, Crescenzi O, D'Ursi AM, D'Alessio G, Tancredi T, Picone D Biochemistry. 2003 Jul 29;42(29):8704-11. PMID:12873130^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Avitabile F, Alfano C, Spadaccini R, Crescenzi O, D'Ursi AM, D'Alessio G, Tancredi T, Picone D. The swapping of terminal arms in ribonucleases: comparison of the solution structure of monomeric bovine seminal and pancreatic ribonucleases. Biochemistry. 2003 Jul 29;42(29):8704-11. PMID:12873130 doi:10.1021/bi0342517

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1qwq"

@@ Line 1: / Line 1: @@
 ==Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease==
 <StructureSection load='1qwq' size='340' side='right' caption='[[1qwq]], [[NMR_Ensembles_of_Models | 16 NMR models]]' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SRN ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9913 BOVIN])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Pancreatic_ribonuclease Pancreatic ribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.27.5 3.1.27.5] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://pdbe.org/1qwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1qwq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1qwq OCA], [http://pdbe.org/1qwq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1qwq RCSB], [http://www.ebi.ac.uk/pdbsum/1qwq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1qwq ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/qw/1qwq_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1qwq

From Proteopedia

Revision as of 08:03, 28 February 2018

Solution structure of the monomeric N67D mutant of Bovine Seminal Ribonuclease

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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