Sandbox Reserved 1373
From Proteopedia
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(→'Green Florescent Protein'==Your Heading Here (maybe something like 'Structure')) |
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{{Sandbox_Reserved_HLSC322}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | {{Sandbox_Reserved_HLSC322}}<!-- PLEASE ADD YOUR CONTENT BELOW HERE --> | ||
| - | == | + | ==Green Fluorescent Protein== |
| - | <scene name='77/777693/Green_fluorescent_protein/1'>Green Fluorescent Protein</scene> is a protein that converts the blue chemiluminescent of aequorin in the jellyfish into green fluorescent light. | + | <scene name='77/777693/Green_fluorescent_protein/1'>Green Fluorescent Protein</scene> is a protein that converts the blue chemiluminescent of aequorin, (a photoprotein) in the Aequorea victoria jellyfish into green fluorescent light. |
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<StructureSection load='1ema' size='340' side='right' caption='Green Fluorescent Protein' scene=''> | <StructureSection load='1ema' size='340' side='right' caption='Green Fluorescent Protein' scene=''> | ||
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| - | You may include any references to papers as in: the use of JSmol in Proteopedia <ref>DOI 10.1002/ijch.201300024</ref> or to the article describing Jmol <ref>PMID:21638687</ref> to the rescue. | ||
== Function == | == Function == | ||
| - | == | + | GFP functions to convert blue chemiluminescent of aequorin into green fluorescent light. Because of its green fluorescence, GFP can also be function as a marker protein when incorporated into a variety of biological molecules and systems. This is useful for gene cloning, because it allows for researchers to determine from its use as a marker if their gene has been successfully incorporated into a cloning vector and replicated in their organism of choice. For example, if the gene coding for insulin was attached on a cloning vector with a gene encoding for GFP, the vector can then be electroporated into a fast growing organism like E.coli. After this, scientists can view if the gene was successfully incorporated based on if the E. coli is fluorescent or not. |
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| + | == Structure == | ||
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| + | Regarding the structure, the protein has a secondary structure of five alpha-helices and one eleven-stranded beta-pleated sheet. GFP is shaped like a barrel, which is from the folding of the <scene name='77/777693/Beta_pleated_sheets/1'>beta pleated sheets</scene>. In addition, one <scene name='77/777693/Alpha_helices/1'>alpha helix</scene> can be found running through the center of the barrel, which contains the fluorophore that allows for fluorescence. | ||
== Relevance == | == Relevance == | ||
Revision as of 16:41, 1 March 2018
| This Sandbox is Reserved from January through July 31, 2018 for use in the course HLSC322: Principles of Genetics and Genomics taught by Genevieve Houston-Ludlam at the University of Maryland, College Park, USA. This reservation includes Sandbox Reserved 1311 through Sandbox Reserved 1430. |
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Green Fluorescent Protein
is a protein that converts the blue chemiluminescent of aequorin, (a photoprotein) in the Aequorea victoria jellyfish into green fluorescent light.
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