2fa2
From Proteopedia
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|PDB= 2fa2 |SIZE=350|CAPTION= <scene name='initialview01'>2fa2</scene>, resolution 2.85Å | |PDB= 2fa2 |SIZE=350|CAPTION= <scene name='initialview01'>2fa2</scene>, resolution 2.85Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SCN:THIOCYANATE ION'>SCN</scene> | + | |LIGAND= <scene name='pdbligand=SCN:THIOCYANATE+ION'>SCN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span> |
|GENE= FUS3, DAC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= FUS3, DAC2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2f49|2F49]], [[2f9g|2F9G]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fa2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fa2 OCA], [http://www.ebi.ac.uk/pdbsum/2fa2 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fa2 RCSB]</span> | ||
}} | }} | ||
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[[Category: Lim, W A.]] | [[Category: Lim, W A.]] | ||
[[Category: Remenyi, A.]] | [[Category: Remenyi, A.]] | ||
| - | [[Category: SCN]] | ||
[[Category: map kinase]] | [[Category: map kinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:59:58 2008'' |
Revision as of 00:00, 31 March 2008
| |||||||
| , resolution 2.85Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | FUS3, DAC2 (Saccharomyces cerevisiae) | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Related: | 2F49, 2F9G
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Fus3 without a peptide from Ste5
Overview
Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active-it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.
About this Structure
2FA2 is a Single protein structure of sequence from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
The Ste5 scaffold allosterically modulates signaling output of the yeast mating pathway., Bhattacharyya RP, Remenyi A, Good MC, Bashor CJ, Falick AM, Lim WA, Science. 2006 Feb 10;311(5762):822-6. Epub 2006 Jan 19. PMID:16424299
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