2fb3
From Proteopedia
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|PDB= 2fb3 |SIZE=350|CAPTION= <scene name='initialview01'>2fb3</scene>, resolution 2.349Å | |PDB= 2fb3 |SIZE=350|CAPTION= <scene name='initialview01'>2fb3</scene>, resolution 2.349Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=5AD:5'-DEOXYADENOSINE'>5AD</scene>, <scene name='pdbligand=GTP:GUANOSINE-5'-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MET:METHIONINE'>MET</scene>, <scene name='pdbligand=POP:PYROPHOSPHATE+2-'>POP</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= MoaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | |GENE= MoaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1tv7|1TV7]], [[2fb2|2FB2]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fb3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fb3 OCA], [http://www.ebi.ac.uk/pdbsum/2fb3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fb3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Haenzelmann, P.]] | [[Category: Haenzelmann, P.]] | ||
[[Category: Schindelin, H.]] | [[Category: Schindelin, H.]] | ||
| - | [[Category: 5AD]] | ||
| - | [[Category: GTP]] | ||
| - | [[Category: MET]] | ||
| - | [[Category: POP]] | ||
| - | [[Category: SF4]] | ||
| - | [[Category: SO4]] | ||
[[Category: 5'-deoxyadenosine]] | [[Category: 5'-deoxyadenosine]] | ||
[[Category: 5'-gtp]] | [[Category: 5'-gtp]] | ||
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[[Category: tim barrel]] | [[Category: tim barrel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:00:18 2008'' |
Revision as of 00:00, 31 March 2008
| |||||||
| , resolution 2.349Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Gene: | MoaA (Staphylococcus aureus) | ||||||
| Related: | 1TV7, 2FB2
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of MoaA in complex with 5'-GTP
Overview
The first step in molybdenum cofactor biosynthesis, the conversion of 5'-GTP to precursor Z, an oxygen-sensitive tetrahydropyranopterin is catalyzed by the S-adenosylmethionine (SAM)-dependent enzyme MoaA and the accessory protein MoaC. This reaction involves the radical-initiated intramolecular rearrangement of the guanine C8 atom. MoaA harbors an N-terminal [4Fe-4S] cluster, which is involved in the reductive cleavage of SAM and generates a 5'-deoxyadenosyl radical (5'-dA*), and a C-terminal [4Fe-4S] cluster presumably involved in substrate binding and/or activation. Biochemical studies identified residues involved in 5'-GTP binding and the determinants of nucleotide specificity. The crystal structure of MoaA in complex with 5'-GTP confirms the biochemical data and provides valuable insights into the subsequent radical reaction. MoaA binds 5'-GTP with high affinity and interacts through its C-terminal [4Fe-4S] cluster with the guanine N1 and N2 atoms, in a yet uncharacterized binding mode. The tightly anchored triphosphate moiety prevents the escape of radical intermediates. This structure also visualizes the L-Met and 5'-dA cleavage products of SAM. Rotation of the 5'-dA ribose and/or conformational changes of the guanosine are proposed to bring the 5'-deoxyadenosyl radical into close proximity of either the ribose C2' and C3' or the guanine C8 carbon atoms leading to hydrogen abstraction.
About this Structure
2FB3 is a Single protein structure of sequence from Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Binding of 5'-GTP to the C-terminal FeS cluster of the radical S-adenosylmethionine enzyme MoaA provides insights into its mechanism., Hanzelmann P, Schindelin H, Proc Natl Acad Sci U S A. 2006 May 2;103(18):6829-34. Epub 2006 Apr 21. PMID:16632608[[Category: [4fe-4s] cluster]]
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