Journal:Acta Cryst F:S1744309112003326
From Proteopedia
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Regions of the current structure that differ from the previously reported examples of hCES1 include Ser365–Asp374, which has very poor density. In the [[2h7c]] structure <scene name='Journal:Acta_Cryst_F:1/Cv/12'>each of the six examples of the section of chain from Ser365 to Asp374 has a different conformation</scene>. The poorly defined density for this same region in rhCES1 is consistent with the observation that <scene name='Journal:Acta_Cryst_F:1/Cv/13'>this loop can adopt multiple conformations despite its participation in forming the twofold interface between chains</scene> (<font color='red'><b>the loop of one subunit is in red</b></font> and <span style="color:orange;background-color:black;font-weight:bold;">the loop of the second subunit is in orange</span>). | Regions of the current structure that differ from the previously reported examples of hCES1 include Ser365–Asp374, which has very poor density. In the [[2h7c]] structure <scene name='Journal:Acta_Cryst_F:1/Cv/12'>each of the six examples of the section of chain from Ser365 to Asp374 has a different conformation</scene>. The poorly defined density for this same region in rhCES1 is consistent with the observation that <scene name='Journal:Acta_Cryst_F:1/Cv/13'>this loop can adopt multiple conformations despite its participation in forming the twofold interface between chains</scene> (<font color='red'><b>the loop of one subunit is in red</b></font> and <span style="color:orange;background-color:black;font-weight:bold;">the loop of the second subunit is in orange</span>). | ||
The current results confirm that rhHCES1 isolated from the ''T. ni'' system is essentially identical to previous examples of this enzyme isolated from cultured insect cells, validating the use of the whole insect system as a source for recombinant proteins in structure determination studies. | The current results confirm that rhHCES1 isolated from the ''T. ni'' system is essentially identical to previous examples of this enzyme isolated from cultured insect cells, validating the use of the whole insect system as a source for recombinant proteins in structure determination studies. | ||
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<references/> | <references/> | ||
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Revision as of 09:25, 12 March 2018
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This page complements a publication in scientific journals and is one of the Proteopedia's Interactive 3D Complement pages. For aditional details please see I3DC.
