2fl1
From Proteopedia
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|PDB= 2fl1 |SIZE=350|CAPTION= <scene name='initialview01'>2fl1</scene>, resolution 2.4Å | |PDB= 2fl1 |SIZE=350|CAPTION= <scene name='initialview01'>2fl1</scene>, resolution 2.4Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene> | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=XYG:[(4Z)-2-[(1Z)-ETHANIMIDOYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>XYG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1xa9|1XA9]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fl1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fl1 OCA], [http://www.ebi.ac.uk/pdbsum/2fl1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fl1 RCSB]</span> | ||
}} | }} | ||
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[[Category: Popov, B.]] | [[Category: Popov, B.]] | ||
[[Category: Tikhonova, T.]] | [[Category: Tikhonova, T.]] | ||
| - | [[Category: SO4]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
[[Category: beta-can fold]] | [[Category: beta-can fold]] | ||
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[[Category: zrfp574]] | [[Category: zrfp574]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:04:12 2008'' |
Revision as of 00:04, 31 March 2008
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| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Related: | 1XA9
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of red fluorescent protein from Zoanthus, zRFP574, at 2.4A resolution
Overview
The three-dimensional structure of the red fluorescent protein (RFP) zRFP574 from the button polyp Zoanthus sp. (two dimers per asymmetric unit, 231 x 4 amino acids) has been determined at 2.4 A resolution in space group C222(1). The crystal structure, refined to a crystallographic R factor of 0.203 (R(free) = 0.249), adopts the beta-barrel fold composed of 11 strands similar to that of the yellow fluorescent protein zYFP538. The zRFP574 chromophore, originating from the protein sequence Asp66-Tyr67-Gly68, has a two-ring structure typical of GFP-like proteins. The bond geometry of residue 66 shows the strong tendency of the corresponding C(alpha) atom to sp(2) hybridization as a consequence of N-acylimine bond formation. The zRFP574 chromophore contains the 65-66 cis-peptide bond characteristic of red fluorescent proteins. The chromophore phenolic ring adopts a cis conformation coplanar with the imidazolinone ring. The crystallographic study has revealed an unexpected chemical feature of the internal chromophore. A decarboxylated side chain of the chromophore-forming residue Asp66 has been observed in the structure. This additional post-translational modification is likely to play a key role in the bathochromic shift of the zRFP574 spectrum.
About this Structure
2FL1 is a Single protein structure of sequence from Zoanthus sp.. Full crystallographic information is available from OCA.
Reference
Structure of a red fluorescent protein from Zoanthus, zRFP574, reveals a novel chromophore., Pletneva N, Pletnev S, Tikhonova T, Popov V, Martynov V, Pletnev V, Acta Crystallogr D Biol Crystallogr. 2006 May;62(Pt 5):527-32. Epub 2006, Apr 19. PMID:16627946
Page seeded by OCA on Mon Mar 31 03:04:12 2008
Categories: Single protein | Zoanthus sp. | Martynov, V. | Pletnev, S. | Pletnev, V. | Pletneva, N. | Popov, B. | Tikhonova, T. | Beta barrel | Beta-can fold | Button polyp | Chromophore | Crystal structure | Emission maximum 574nm | Fluorescent marker | Intersubunit interface | Red fluorescent protein | Tightly packed tetramer | Zrfp574
