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1te6

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Revision as of 08:04, 14 March 2018

Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom

Structural highlights

1te6 is a 2 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , ,
Gene:	ENO2 (HUMAN)
Activity:	Phosphopyruvate hydratase, with EC number 4.2.1.11
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ENOG_HUMAN] Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Human neuron-specific enolase (NSE) or isozyme gamma has been expressed with a C-terminal His-tag in Escherichia coli. The enzyme has been purified, crystallized and its crystal structure determined. In the crystals the enzyme forms the asymmetric complex NSE x Mg2 x SO4/NSE x Mg x Cl, where "/" separates the dimer subunits. The subunit that contains the sulfate (or phosphate) ion and two magnesium ions is in the closed conformation observed in enolase complexes with the substrate or its analogues; the other subunit is in the open conformation observed in enolase subunits without bound substrate or analogues. This indicates negative cooperativity for ligand binding between subunits. Electrostatic charge differences between isozymes alpha and gamma, -19 at physiological pH, are concentrated in the regions of the molecular surface that are negatively charged in alpha, i.e. surface areas negatively charged in alpha are more negatively charged in gamma, while areas that are neutral or positively charged tend to be charge-conserved.

Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase.,Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L J Mol Biol. 2004 Aug 20;341(4):1015-21. PMID:15289101^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Chai G, Brewer JM, Lovelace LL, Aoki T, Minor W, Lebioda L. Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase. J Mol Biol. 2004 Aug 20;341(4):1015-21. PMID:15289101 doi:10.1016/j.jmb.2004.05.068

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1te6"

@@ Line 1: / Line 1: @@
 ==Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom==
 <StructureSection load='1te6' size='340' side='right' caption='[[1te6]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">ENO2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Phosphopyruvate_hydratase Phosphopyruvate hydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.11 4.2.1.11] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1te6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1te6 OCA], [http://pdbe.org/1te6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1te6 RCSB], [http://www.ebi.ac.uk/pdbsum/1te6 PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1te6 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1te6 OCA], [http://pdbe.org/1te6 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1te6 RCSB], [http://www.ebi.ac.uk/pdbsum/1te6 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1te6 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1te6_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/te/1te6_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1te6

From Proteopedia

Revision as of 08:04, 14 March 2018

Crystal Structure of Human Neuron Specific Enolase at 1.8 angstrom

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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