2fx3
From Proteopedia
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|PDB= 2fx3 |SIZE=350|CAPTION= <scene name='initialview01'>2fx3</scene>, resolution 3.400Å | |PDB= 2fx3 |SIZE=350|CAPTION= <scene name='initialview01'>2fx3</scene>, resolution 3.400Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fx3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fx3 OCA], [http://www.ebi.ac.uk/pdbsum/2fx3 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fx3 RCSB]</span> | ||
}} | }} | ||
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[[Category: Jurnak, F.]] | [[Category: Jurnak, F.]] | ||
[[Category: Moeller, R.]] | [[Category: Moeller, R.]] | ||
| - | [[Category: GDP]] | ||
| - | [[Category: MG]] | ||
[[Category: ef-tu]] | [[Category: ef-tu]] | ||
[[Category: merohedral twinning]] | [[Category: merohedral twinning]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:08:49 2008'' |
Revision as of 00:08, 31 March 2008
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| , resolution 3.400Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set
Overview
Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120 degrees . To determine whether an inhibitor was present in the crystal, a poor-quality X-ray diffraction data set had to be processed. The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP.
About this Structure
2FX3 is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Solving the structure of Escherichia coli elongation factor Tu using a twinned data set., Heffron SE, Moeller R, Jurnak F, Acta Crystallogr D Biol Crystallogr. 2006 Apr;62(Pt 4):433-8. Epub 2006, Mar 18. PMID:16552145
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