2fxl
From Proteopedia
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|PDB= 2fxl |SIZE=350|CAPTION= <scene name='initialview01'>2fxl</scene>, resolution 1.76Å | |PDB= 2fxl |SIZE=350|CAPTION= <scene name='initialview01'>2fxl</scene>, resolution 1.76Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=2AL:1-(2,5-DIOXO-2,5-DIHYDRO-1H-IMIDAZOL-4-YL)UREA'>2AL</scene> | + | |LIGAND= <scene name='pdbligand=2AL:1-(2,5-DIOXO-2,5-DIHYDRO-1H-IMIDAZOL-4-YL)UREA'>2AL</scene>, <scene name='pdbligand=SAC:N-ACETYL-SERINE'>SAC</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Urate_oxidase Urate oxidase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.7.3.3 1.7.3.3] </span> |
|GENE= uaZ, uox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 Aspergillus flavus]) | |GENE= uaZ, uox ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=5059 Aspergillus flavus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1r4s|1R4S]], [[1r4u|1R4U]], [[1r51|1R51]], [[1r56|1R56]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fxl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fxl OCA], [http://www.ebi.ac.uk/pdbsum/2fxl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2fxl RCSB]</span> | ||
}} | }} | ||
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[[Category: H, N Colloc.]] | [[Category: H, N Colloc.]] | ||
[[Category: Prange, T.]] | [[Category: Prange, T.]] | ||
| - | [[Category: 2AL]] | ||
[[Category: allantoin]] | [[Category: allantoin]] | ||
[[Category: dimeric barrel]] | [[Category: dimeric barrel]] | ||
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[[Category: uric acid degradation]] | [[Category: uric acid degradation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:09:00 2008'' |
Revision as of 00:09, 31 March 2008
| |||||||
| , resolution 1.76Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | uaZ, uox (Aspergillus flavus) | ||||||
| Activity: | Urate oxidase, with EC number 1.7.3.3 | ||||||
| Related: | 1R4S, 1R4U, 1R51, 1R56
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Urate oxidase from aspergillus flavus complexed with allantoin
Overview
Urate oxidase from Aspergillus flavus catalyzes the degradation of uric acid to [S]-allantoin through 5-hydroxyisourate as a metastable intermediate. The second degradation step is thought either catalyzed by another specific enzyme, or spontaneous. The structure of the enzyme was known at high resolution by X-ray diffraction of I222 crystals complexed with a purine-type inhibitor (8-azaxanthin). Analyzing the X-ray structure of urate oxidase treated with an excess of urate, the natural substrate, shows unexpectedly that the active site recaptures [S]-allantoin from the racemic end product of a second degradation step.
About this Structure
2FXL is a Single protein structure of sequence from Aspergillus flavus. Full crystallographic information is available from OCA.
Reference
Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase., Gabison L, Chiadmi M, Colloc'h N, Castro B, El Hajji M, Prange T, FEBS Lett. 2006 Apr 3;580(8):2087-91. Epub 2006 Mar 10. PMID:16545381
Page seeded by OCA on Mon Mar 31 03:09:00 2008
