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2g0k
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= ncsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1897 Streptomyces carzinostaticus]) | |GENE= ncsA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1897 Streptomyces carzinostaticus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1j5h|1J5H]], [[1j5i|1J5I]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g0k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g0k OCA], [http://www.ebi.ac.uk/pdbsum/2g0k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g0k RCSB]</span> | ||
}} | }} | ||
| Line 29: | Line 32: | ||
[[Category: igg fold]] | [[Category: igg fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:10:11 2008'' |
Revision as of 00:10, 31 March 2008
| |||||||
| Gene: | ncsA (Streptomyces carzinostaticus) | ||||||
| Related: | 1J5H, 1J5I
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Solution Structure of Neocarzinostatin Apo-Protein
Overview
Neocarzinostatin (NCS) is a 1:1 complex of an enediyne chromophore (NCSChrom), non-covalently bound to an 11 kDa protein (apoNCS). We are exploring apoNCS as a generic protein system for sequestering small molecules for therapeutic applications. Here, we disclose a new flavone ligand 1 for apoNCS and present a high-resolution NMR structure of this ligand bound to apoNCS. This is the first high-resolution structure of a completely non-cognate ligand bound to the apoNCS protein. This work provides unambiguous evidence that a completely new class of ligand can bind specifically to apoNCS. Furthermore, the mode of binding is different than that of the naphthoate-based ligands, and for such a simple hydrophobic compound, the new ligand surprisingly binds specifically. This work indicates that apo-Neocarzinostatin has multiple selective and distinct binding modes for small-molecule cargo.
About this Structure
2G0K is a Single protein structure of sequence from Streptomyces carzinostaticus. Full crystallographic information is available from OCA.
Reference
Synthetic ligands for apo-neocarzinostatin., Caddick S, Muskett FW, Stoneman RG, Woolfson DN, J Am Chem Soc. 2006 Apr 5;128(13):4204-5. PMID:16568976
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