1u02

From Proteopedia

(Difference between revisions)

Revision as of 09:05, 21 March 2018

Crystal structure of trehalose-6-phosphate phosphatase related protein

Structural highlights

1u02 is a 1 chain structure with sequence from "thermoplasma_acidophila"_(sic)_darland_et_al._1970 "thermoplasma acidophila" (sic) darland et al. 1970. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
NonStd Res:
Gene:	Ta1209 ("Thermoplasma acidophila" (sic) Darland et al. 1970)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT, TOPSAN

Function

[OTSBH_THEAC] Removes the phosphate from trehalose 6-phosphate (Tre6P) to produce free trehalose. Also catalyzes the dephosphorylation of para-nitrophenyl phosphate (pNPP), but with lesser efficiency (in vitro).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

We report here the crystal structure of a trehalose-6-phosphate phosphatase-related protein (T6PP) from Thermoplasma acidophilum, TA1209, determined by the dual-wavelength anomalous diffraction (DAD) method. T6PP is a member of the haloacid dehalogenase (HAD) superfamily with significant sequence homology with trehalose-6-phosphate phosphatase, phosphoserine phosphatase, P-type ATPases and other members of the family. T6PP possesses a core domain of known alpha/beta-hydrolase fold, characteristic of the HAD family, and a cap domain, with a tertiary fold consisting of a four-stranded beta-sheet with two alpha-helices on one side of the sheet. An active-site magnesium ion and a glycerol molecule bound at the interface between the two domains provide insight into the mode of substrate binding by T6PP. A trehalose-6-phosphate molecule modeled into a cage formed by the two domains makes favorable interactions with the protein molecule. We have confirmed that T6PP is a trehalose phosphatase from amino acid sequence, three-dimensional structure, and biochemical assays.

Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications.,Rao KN, Kumaran D, Seetharaman J, Bonanno JB, Burley SK, Swaminathan S Protein Sci. 2006 Jul;15(7):1735-44. PMID:16815921^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rao KN, Kumaran D, Seetharaman J, Bonanno JB, Burley SK, Swaminathan S. Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications. Protein Sci. 2006 Jul;15(7):1735-44. PMID:16815921 doi:15/7/1735
↑ Rao KN, Kumaran D, Seetharaman J, Bonanno JB, Burley SK, Swaminathan S. Crystal structure of trehalose-6-phosphate phosphatase-related protein: biochemical and biological implications. Protein Sci. 2006 Jul;15(7):1735-44. PMID:16815921 doi:15/7/1735

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1u02"

@@ Line 1: / Line 1: @@
 ==Crystal structure of trehalose-6-phosphate phosphatase related protein==
 <StructureSection load='1u02' size='340' side='right' caption='[[1u02]], [[Resolution|resolution]] 1.92&Aring;' scene=''>
@@ Line 6: / Line 7: @@
 <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ta1209 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2303 "Thermoplasma acidophila" (sic) Darland et al. 1970])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u02 OCA], [http://pdbe.org/1u02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u02 RCSB], [http://www.ebi.ac.uk/pdbsum/1u02 PDBsum], [http://www.topsan.org/Proteins/NYSGXRC/1u02 TOPSAN]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1u02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1u02 OCA], [http://pdbe.org/1u02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1u02 RCSB], [http://www.ebi.ac.uk/pdbsum/1u02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1u02 ProSAT], [http://www.topsan.org/Proteins/NYSGXRC/1u02 TOPSAN]</span></td></tr>
 </table>
 == Function ==
@@ Line 14: / Line 15: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u02_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/u0/1u02_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

1u02

From Proteopedia

Revision as of 09:05, 21 March 2018

Crystal structure of trehalose-6-phosphate phosphatase related protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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