2gmr
From Proteopedia
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|PDB= 2gmr |SIZE=350|CAPTION= <scene name='initialview01'>2gmr</scene>, resolution 2.50Å | |PDB= 2gmr |SIZE=350|CAPTION= <scene name='initialview01'>2gmr</scene>, resolution 2.50Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=BCL:BACTERIOCHLOROPHYLL+A'>BCL</scene>, <scene name='pdbligand=BPH:BACTERIOPHEOPHYTIN+A'>BPH</scene>, <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>, <scene name='pdbligand=LDA:LAURYL+DIMETHYLAMINE-N-OXIDE'>LDA</scene>, <scene name='pdbligand=SPN:SPEROIDENONE'>SPN</scene>, <scene name='pdbligand=U10:UBIQUINONE-10'>U10</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= pufL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), pufM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), puhA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]) | |GENE= pufL ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), pufM ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]), puhA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1063 Rhodobacter sphaeroides]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gmr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gmr OCA], [http://www.ebi.ac.uk/pdbsum/2gmr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gmr RCSB]</span> | ||
}} | }} | ||
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[[Category: Hofmann, E.]] | [[Category: Hofmann, E.]] | ||
[[Category: Stachnik, J M.]] | [[Category: Stachnik, J M.]] | ||
| - | [[Category: BCL]] | ||
| - | [[Category: BPH]] | ||
| - | [[Category: FE2]] | ||
| - | [[Category: LDA]] | ||
| - | [[Category: SPN]] | ||
| - | [[Category: U10]] | ||
[[Category: electron transport]] | [[Category: electron transport]] | ||
[[Category: integral membrane protein]] | [[Category: integral membrane protein]] | ||
| Line 40: | Line 37: | ||
[[Category: x-ray crystallography]] | [[Category: x-ray crystallography]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:18:44 2008'' |
Revision as of 00:18, 31 March 2008
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| , resolution 2.50Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , , , | ||||||
| Gene: | pufL (Rhodobacter sphaeroides), pufM (Rhodobacter sphaeroides), puhA (Rhodobacter sphaeroides) | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Photosynthetic reaction center mutant from Rhodobacter sphaeroides with Asp L210 replaced with Asn
Overview
The reaction center (RC) of Rhodobacter sphaeroides uses light energy to reduce and protonate a quinone molecule, QB (the secondary quinone electron acceptor), to form quinol, QBH2. Asp210 in the L-subunit has been shown to be a catalytic residue in this process. Mutation of Asp210 to Asn leads to a deceleration of reoxidation of QA- in the QA-QB --> QAQB- transition. Here we determined the structure of the Asp210 to Asn mutant to 2.5 A and show that there are no major structural differences as compared to the wild-type protein. We found QB in the distal position and a chain of water molecules between Asn210 and QB. Using time-resolved Fourier transform infrared (trFTIR) spectroscopy, we characterized the molecular reaction mechanism of this mutant. We found that QB- formation precedes QA- oxidation even more pronounced than in the wild-type reaction center. Continuum absorbance changes indicate deprotonation of a protonated water cluster, most likely of the water chain between Asn210 and QB. A detailed analysis of wild-type structures revealed a highly conserved water chain between Asp210 or Glu210 and QB in Rb. sphaeroides and Rhodopseudomonas viridis, respectively.
About this Structure
2GMR is a Protein complex structure of sequences from Rhodobacter sphaeroides. Full crystallographic information is available from OCA.
Reference
Proton uptake in the reaction center mutant L210DN from Rhodobacter sphaeroides via protonated water molecules., Hermes S, Stachnik JM, Onidas D, Remy A, Hofmann E, Gerwert K, Biochemistry. 2006 Nov 21;45(46):13741-9. PMID:17105193
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