This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5xeo
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of a hydrogen sulfide-producing enzyme (Fn1220) from Fusobacterium nucleatum== | |
| + | <StructureSection load='5xeo' size='340' side='right' caption='[[5xeo]], [[Resolution|resolution]] 2.03Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[5xeo]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XEO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XEO FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PLP:PYRIDOXAL-5-PHOSPHATE'>PLP</scene></td></tr> | ||
| + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5xem|5xem]], [[5xen|5xen]]</td></tr> | ||
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Cysteine_synthase Cysteine synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.47 2.5.1.47] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xeo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xeo OCA], [http://pdbe.org/5xeo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xeo RCSB], [http://www.ebi.ac.uk/pdbsum/5xeo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xeo ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Hydrogen sulfide produced by oral bacteria is responsible for oral malodour. Two homologous hydrogen sulfide-producing enzymes, Fn1220 and Cdl, from Fusobacterium nucleatum (which actively produces hydrogen sulfide) were overproduced, purified and crystallized. X-ray diffraction data were collected from the crystals using a synchrotron-radiation source. The Fn1220 crystal belonged to tetragonal space group P4(1)2(1)2 or P4(3)2(1)2 (unit-cell parameters a=b=116.8, c=99.2 A) and the Cdl crystal belonged to monoclinic space group P2(1) (unit-cell parameters a=84.9, b=70.9, c=87.6 A, beta=90.3 degrees ). | ||
| - | + | Purification, crystallization and preliminary X-ray analysis of two hydrogen sulfide-producing enzymes from Fusobacterium nucleatum.,Kezuka Y, Abe N, Yoshida Y, Nonaka T Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Dec 1;68(Pt 12):1507-10., doi: 10.1107/S1744309112042546. Epub 2012 Nov 14. PMID:23192034<ref>PMID:23192034</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 5xeo" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Cysteine synthase]] | ||
[[Category: Kezuka, Y]] | [[Category: Kezuka, Y]] | ||
| - | [[Category: Yoshida, Y]] | ||
[[Category: Nonaka, T]] | [[Category: Nonaka, T]] | ||
| + | [[Category: Yoshida, Y]] | ||
| + | [[Category: Beta-replacement reaction]] | ||
| + | [[Category: Hydrogen sulfide]] | ||
| + | [[Category: L-cysteine]] | ||
| + | [[Category: Lanthionine]] | ||
| + | [[Category: Periodontal disease]] | ||
| + | [[Category: Pyridoxal 5'-phosphate dependent enzyme]] | ||
| + | [[Category: Transferase]] | ||
Revision as of 13:46, 11 April 2018
Crystal structure of a hydrogen sulfide-producing enzyme (Fn1220) from Fusobacterium nucleatum
| |||||||||||
