2gwl
From Proteopedia
| Line 7: | Line 7: | ||
|ACTIVITY= | |ACTIVITY= | ||
|GENE= mkaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | |GENE= mkaA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=602 Salmonella typhimurium]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2gwm|2GWM]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gwl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gwl OCA], [http://www.ebi.ac.uk/pdbsum/2gwl PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gwl RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Margarit, S M.]] | [[Category: Margarit, S M.]] | ||
[[Category: Stebbins, C E.]] | [[Category: Stebbins, C E.]] | ||
| - | [[Category: NAD]] | ||
[[Category: adp-ribosyltransferase]] | [[Category: adp-ribosyltransferase]] | ||
[[Category: salmonella]] | [[Category: salmonella]] | ||
[[Category: spvb]] | [[Category: spvb]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:22:40 2008'' |
Revision as of 00:22, 31 March 2008
| |||||||
| , resolution 1.900Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | mkaA (Salmonella typhimurium) | ||||||
| Related: | 2GWM
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the Salmonella SpvB ATR Domain in complex with NADH
Overview
Salmonella spp. require the ADP-ribosyltransferase activity of the SpvB protein for intracellular growth and systemic virulence. SpvB covalently modifies actin, causing cytoskeletal disruption and apoptosis. We report here the crystal structure of the catalytic domain of SpvB, and we show by mass spectrometric analysis that SpvB modifies actin at Arg177, inhibiting its ATPase activity. We also describe two crystal structures of SpvB-modified, polymerization-deficient actin. These structures reveal that ADP-ribosylation does not lead to dramatic conformational changes in actin, suggesting a model in which this large family of toxins inhibits actin polymerization primarily through steric disruption of intrafilament contacts.
About this Structure
2GWL is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
A steric antagonism of actin polymerization by a salmonella virulence protein., Margarit SM, Davidson W, Frego L, Stebbins CE, Structure. 2006 Aug;14(8):1219-29. PMID:16905096
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