2gy9
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2gy9 |SIZE=350|CAPTION= <scene name='initialview01'>2gy9</scene> | |PDB= 2gy9 |SIZE=350|CAPTION= <scene name='initialview01'>2gy9</scene> | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=A:ADENOSINE-5'-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=C:CYTIDINE-5'-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5'-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5'-MONOPHOSPHATE'>U</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2gya|2GYA]], [[2gyb|2GYB]], [[2gyc|2GYC]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gy9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gy9 OCA], [http://www.ebi.ac.uk/pdbsum/2gy9 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gy9 RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Frank, J.]] | [[Category: Frank, J.]] | ||
[[Category: Mitra, K.]] | [[Category: Mitra, K.]] | ||
| - | [[Category: | + | [[Category: elongation arrest]] |
| + | [[Category: nascent chain]] | ||
| + | [[Category: polypeptide exit tunnel]] | ||
| + | [[Category: ribosome]] | ||
| + | [[Category: rna world]] | ||
| + | [[Category: secm]] | ||
| + | [[Category: signal transduction]] | ||
| + | [[Category: translocation]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:22:54 2008'' |
Revision as of 00:22, 31 March 2008
| |||||||
| Ligands: | , , , | ||||||
| Related: | 2GYA, 2GYB, 2GYC
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the 30S subunit of a pre-translocational E. coli ribosome obtained by fitting atomic models for RNA and protein components into cryo-EM map EMD-1056
Overview
In E. coli, the SecM nascent polypeptide causes elongation arrest, while interacting with 23S RNA bases A2058 and A749-753 in the exit tunnel of the large ribosomal subunit. We compared atomic models fitted by real-space refinement into cryo-electron microscopy reconstructions of a pretranslocational and SecM-stalled E. coli ribosome complex. A cascade of RNA rearrangements propagates from the exit tunnel throughout the large subunit, affecting intersubunit bridges and tRNA positions, which in turn reorient small subunit RNA elements. Elongation arrest could result from the inhibition of mRNA.(tRNAs) translocation, E site tRNA egress, and perhaps translation factor activation at the GTPase-associated center. Our study suggests that the specific secondary and tertiary arrangement of ribosomal RNA provides the basis for internal signal transduction within the ribosome. Thus, the ribosome may itself have the ability to regulate its progression through translation by modulating its structure and consequently its receptivity to activation by cofactors.
About this Structure
2GY9 is a Protein complex structure of sequences from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Elongation arrest by SecM via a cascade of ribosomal RNA rearrangements., Mitra K, Schaffitzel C, Fabiola F, Chapman MS, Ban N, Frank J, Mol Cell. 2006 May 19;22(4):533-43. PMID:16713583
Page seeded by OCA on Mon Mar 31 03:22:54 2008
