2gyd
From Proteopedia
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|PDB= 2gyd |SIZE=350|CAPTION= <scene name='initialview01'>2gyd</scene>, resolution 1.720Å | |PDB= 2gyd |SIZE=350|CAPTION= <scene name='initialview01'>2gyd</scene>, resolution 1.720Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene> | + | |LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=DFE:2-(1,1-DIFLUOROETHOXY)-1,1,1-TRIFLUOROETHANE'>DFE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gyd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gyd OCA], [http://www.ebi.ac.uk/pdbsum/2gyd PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gyd RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Loll, P J.]] | [[Category: Loll, P J.]] | ||
[[Category: Rossi, M J.]] | [[Category: Rossi, M J.]] | ||
| - | [[Category: CD]] | ||
| - | [[Category: DFE]] | ||
[[Category: anesthetic]] | [[Category: anesthetic]] | ||
[[Category: helical bundle]] | [[Category: helical bundle]] | ||
[[Category: isoflurane]] | [[Category: isoflurane]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:22:57 2008'' |
Revision as of 00:22, 31 March 2008
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| , resolution 1.720Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Complex of equine apoferritin with the H-diaziflurane photolabeling reagent
Overview
The difficulty in obtaining binding target and site information for low-affinity drugs, like the inhaled anesthetics, has limited identification of their molecular effectors. Because such information can be provided by photoactive analogues, we designed, synthesized, and characterized a novel diazirnyl haloether that closely mimics isoflurane, the most widely used clinical general anesthetic. This compound, H-diaziflurane, is a nontoxic, potent anesthetic that potentiates GABA-gated ion channels in primary cultures of hippocampal neurons. Calorimetric and structural characterizations show that H-diaziflurane binds a model anesthetic host protein with similar energetics as isoflurane and forms photoadducts with residues lining the isoflurane binding site. H-diaziflurane will be immediately useful for identifying targets and sites important for the molecular pharmacology of the inhaled haloether anesthetics.
About this Structure
2GYD is a Single protein structure of sequence from Equus caballus. Full crystallographic information is available from OCA.
Reference
Photoactive analogues of the haloether anesthetics provide high-resolution features from low-affinity interactions., Xi J, Liu R, Rossi MJ, Yang J, Loll PJ, Dailey WP, Eckenhoff RG, ACS Chem Biol. 2006 Jul 21;1(6):377-84. PMID:17163775
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