3ryk
From Proteopedia
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ryk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ryk OCA], [http://pdbe.org/3ryk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ryk RCSB], [http://www.ebi.ac.uk/pdbsum/3ryk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ryk ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3ryk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3ryk OCA], [http://pdbe.org/3ryk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3ryk RCSB], [http://www.ebi.ac.uk/pdbsum/3ryk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3ryk ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The exosporium layer of Bacillus anthracis spores is rich in L-rhamnose, a common bacterial cell-wall component, which often contributes to the virulence of pathogens by increasing their adherence and immune evasion. The biosynthetic pathway used to form the activated L-rhamnose donor dTDP-L-rhamnose consists of four enzymes (RfbA, RfbB, RfbC and RfbD) and is an attractive drug target because there are no homologs in mammals. It was found that co-purifying and screening RfbC (dTDP-6-deoxy-D-xylo-4-hexulose 3,5-epimerase) from B. anthracis in the presence of the other three B. anthracis enzymes of the biosynthetic pathway yielded crystals that were suitable for data collection. RfbC crystallized as a dimer and its structure was determined at 1.63 A resolution. Two different ligands were bound in the protein structure: pyrophosphate in the active site of one monomer and dTDP in the other monomer. A structural comparison with RfbC homologs showed that the key active-site residues are conserved across kingdoms. | ||
| + | |||
| + | Structure of the Bacillus anthracis dTDP-L-rhamnose-biosynthetic enzyme dTDP-4-dehydrorhamnose 3,5-epimerase (RfbC).,Shornikov A, Tran H, Macias J, Halavaty AS, Minasov G, Anderson WF, Kuhn ML Acta Crystallogr F Struct Biol Commun. 2017 Dec 1;73(Pt 12):664-671. doi:, 10.1107/S2053230X17015849. Epub 2017 Nov 10. PMID:29199987<ref>PMID:29199987</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 3ryk" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[RmlC|RmlC]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
Revision as of 06:06, 18 April 2018
1.63 Angstrom resolution crystal structure of dTDP-4-dehydrorhamnose 3,5-epimerase (rfbC) from Bacillus anthracis str. Ames with TDP and PPi bound
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Categories: DTDP-4-dehydrorhamnose 3,5-epimerase | Anderson, W F | Structural genomic | Halavaty, A S | Kuhn, M | Kwon, K | Minasov, G | Shuvalova, L | 5-epimerase | Dtdp-4-dehydrorhamnose 3 | Infectious disease | Isomerase | Jelly roll-like topology | Rhamnose pathway | The 3 and 5 positions of the sugar ring | The enzyme epimerizes at two carbon center
