5f5t
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
<StructureSection load='5f5t' size='340' side='right' caption='[[5f5t]], [[Resolution|resolution]] 2.55Å' scene=''> | <StructureSection load='5f5t' size='340' side='right' caption='[[5f5t]], [[Resolution|resolution]] 2.55Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5f5t]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F5T FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5f5t]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5T OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F5T FirstGlance]. <br> |
| - | </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5t OCA], [http://pdbe.org/5f5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5t RCSB], [http://www.ebi.ac.uk/pdbsum/5f5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5t ProSAT]</span></td></tr> | + | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CTHT_0013190 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD]), CTHT_0069660 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr> |
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f5t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5t OCA], [http://pdbe.org/5f5t PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5t RCSB], [http://www.ebi.ac.uk/pdbsum/5f5t PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5t ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | The spliceosomal B complex-specific protein Prp38 forms a complex with the intrinsically unstructured proteins MFAP1 and Snu23. Our binding and crystal structure analyses show that MFAP1 and Snu23 contact Prp38 via ER/K motif-stabilized single alpha helices, which have previously been recognized only as rigid connectors or force springs between protein domains. A variant of the Prp38-binding single alpha helix of MFAP1, in which ER/K motifs not involved in Prp38 binding were mutated, was less alpha-helical in isolation and showed a reduced Prp38 affinity, with opposing tendencies in interaction enthalpy and entropy. Our results indicate that the strengths of single alpha helix-based interactions can be tuned by the degree of helix stabilization in the unbound state. MFAP1, Snu23, and several other spliceosomal proteins contain multiple regions that likely form single alpha helices via which they might tether several binding partners and act as intermittent scaffolds that facilitate remodeling steps during assembly of an active spliceosome. | ||
| + | |||
| + | Scaffolding in the Spliceosome via Single alpha Helices.,Ulrich AKC, Seeger M, Schutze T, Bartlick N, Wahl MC Structure. 2016 Nov 1;24(11):1972-1983. doi: 10.1016/j.str.2016.09.007. Epub 2016, Oct 20. PMID:27773687<ref>PMID:27773687</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5f5t" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Chatd]] | ||
[[Category: Ulrich, A K.C]] | [[Category: Ulrich, A K.C]] | ||
[[Category: Wahl, M C]] | [[Category: Wahl, M C]] | ||
Revision as of 06:21, 18 April 2018
Crystal structure of the Prp38-MFAP1 complex of Chaetomium thermophilum
| |||||||||||
