5vim

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<StructureSection load='5vim' size='340' side='right' caption='[[5vim]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
<StructureSection load='5vim' size='340' side='right' caption='[[5vim]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
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<table><tr><td colspan='2'>[[5vim]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VIM FirstGlance]. <br>
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<table><tr><td colspan='2'>[[5vim]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Zikv Zikv]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5VIM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5VIM FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SAM:S-ADENOSYLMETHIONINE'>SAM</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vim OCA], [http://pdbe.org/5vim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vim RCSB], [http://www.ebi.ac.uk/pdbsum/5vim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vim ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5vim FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5vim OCA], [http://pdbe.org/5vim PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5vim RCSB], [http://www.ebi.ac.uk/pdbsum/5vim PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5vim ProSAT]</span></td></tr>
</table>
</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Two nonstructural proteins encoded by Zika virus strain MR766 RNA, a methyltransferase and a helicase, were crystallized and their structures were solved and refined at 2.10 and 2.01 A resolution, respectively. The NS5 methyltransferase contains a bound S-adenosyl-L-methionine (SAM) co-substrate. The NS3 helicase is in the apo form. Comparison with published crystal structures of the helicase in the apo, nucleotide-bound and single-stranded RNA (ssRNA)-bound states suggests that binding of ssRNA to the helicase may occur through conformational selection rather than induced fit.
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Crystal structures of the methyltransferase and helicase from the ZIKA 1947 MR766 Uganda strain.,Bukrejewska M, Derewenda U, Radwanska M, Engel DA, Derewenda ZS Acta Crystallogr D Struct Biol. 2017 Sep 1;73(Pt 9):767-774. doi:, 10.1107/S2059798317010737. Epub 2017 Aug 15. PMID:28876240<ref>PMID:28876240</ref>
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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</div>
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<div class="pdbe-citations 5vim" style="background-color:#fffaf0;"></div>
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== References ==
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<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
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[[Category: Zikv]]
[[Category: Bukrejewska, M]]
[[Category: Bukrejewska, M]]
[[Category: Derewenda, U]]
[[Category: Derewenda, U]]

Revision as of 06:37, 18 April 2018

Crystal structure of the Zika virus NS5 methyltransferase.

5vim, resolution 2.10Å

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