2h7v
From Proteopedia
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|PDB= 2h7v |SIZE=350|CAPTION= <scene name='initialview01'>2h7v</scene>, resolution 2.600Å | |PDB= 2h7v |SIZE=350|CAPTION= <scene name='initialview01'>2h7v</scene>, resolution 2.600Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=GDP:GUANOSINE-5'-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= MIG5, RAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ypkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 Yersinia pseudotuberculosis]) | |GENE= MIG5, RAC1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), ypkA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=633 Yersinia pseudotuberculosis]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2h7o|2H7O]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h7v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h7v OCA], [http://www.ebi.ac.uk/pdbsum/2h7v PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h7v RCSB]</span> | ||
}} | }} | ||
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[[Category: Prehna, G.]] | [[Category: Prehna, G.]] | ||
[[Category: Stebbins, C E.]] | [[Category: Stebbins, C E.]] | ||
| - | [[Category: GDP]] | ||
| - | [[Category: MG]] | ||
[[Category: gdi]] | [[Category: gdi]] | ||
[[Category: gtpase]] | [[Category: gtpase]] | ||
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[[Category: ypka]] | [[Category: ypka]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:42 2008'' |
Revision as of 00:26, 31 March 2008
| |||||||
| , resolution 2.600Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | MIG5, RAC1 (Homo sapiens), ypkA (Yersinia pseudotuberculosis) | ||||||
| Related: | 2H7O
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Co-crystal structure of YpkA-Rac1
Overview
Yersinia spp. cause gastroenteritis and the plague, representing historically devastating pathogens that are currently an important biodefense and antibiotic resistance concern. A critical virulence determinant is the Yersinia protein kinase A, or YpkA, a multidomain protein that disrupts the eukaryotic actin cytoskeleton. Here we solve the crystal structure of a YpkA-Rac1 complex and find that YpkA possesses a Rac1 binding domain that mimics host guanidine nucleotide dissociation inhibitors (GDIs) of the Rho GTPases. YpkA inhibits nucleotide exchange in Rac1 and RhoA, and mutations that disrupt the YpkA-GTPase interface abolish this activity in vitro and impair in vivo YpkA-induced cytoskeletal disruption. In cell culture experiments, the kinase and the GDI domains of YpkA act synergistically to promote cytoskeletal disruption, and a Y. pseudotuberculosis mutant lacking YpkA GDI activity shows attenuated virulence in a mouse infection assay. We conclude that virulence in Yersinia depends strongly upon mimicry of host GDI proteins by YpkA.
About this Structure
2H7V is a Protein complex structure of sequences from Homo sapiens and Yersinia pseudotuberculosis. Full crystallographic information is available from OCA.
Reference
Yersinia virulence depends on mimicry of host Rho-family nucleotide dissociation inhibitors., Prehna G, Ivanov MI, Bliska JB, Stebbins CE, Cell. 2006 Sep 8;126(5):869-80. PMID:16959567
Page seeded by OCA on Mon Mar 31 03:26:42 2008
Categories: Homo sapiens | Protein complex | Yersinia pseudotuberculosis | Bliska, J B. | Ivanov, M. | Prehna, G. | Stebbins, C E. | Gdi | Gtpase | Rac1 | Yersinia | Yopo | Ypka
