2h8e
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= rusA, rus ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | |GENE= rusA, rus ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2h8c|2H8C]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h8e OCA], [http://www.ebi.ac.uk/pdbsum/2h8e PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h8e RCSB]</span> | ||
}} | }} | ||
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[[Category: resolvase]] | [[Category: resolvase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:26:52 2008'' |
Revision as of 00:26, 31 March 2008
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| , resolution 1.20Å | |||||||
|---|---|---|---|---|---|---|---|
| Gene: | rusA, rus (Escherichia coli) | ||||||
| Related: | 2H8C
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure RusA D70N
Overview
We have determined the structure of a catalytically inactive D70N variant of the Escherichia coli RusA resolvase bound to a duplex DNA substrate that reveals critical protein-DNA interactions and permits a much clearer understanding of the interaction of the enzyme with a Holliday junction (HJ). The RusA enzyme cleaves HJs, the fourway DNA branchpoints formed by homologous recombination, by introducing symmetrical cuts in the phosphodiester backbone in a Mg2+ dependent reaction. Although, RusA shows a high level of selectivity for DNA junctions, preferring to bind fourway junctions over other substrates in vitro, it has also been shown to have appreciable affinity for duplex DNA. However, RusA does not show DNA cleavage activity with duplex substrates. Our structure suggests the possible basis for structural selectivity as well as sources of the sequence specificity observed for DNA cleavage by RusA.
About this Structure
2H8E is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
RusA Holliday junction resolvase: DNA complex structure--insights into selectivity and specificity., Macmaster R, Sedelnikova S, Baker PJ, Bolt EL, Lloyd RG, Rafferty JB, Nucleic Acids Res. 2006;34(19):5577-84. Epub 2006 Oct 5. PMID:17028102
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