5jrp
From Proteopedia
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<StructureSection load='5jrp' size='340' side='right' caption='[[5jrp]], [[Resolution|resolution]] 2.00Å' scene=''> | <StructureSection load='5jrp' size='340' side='right' caption='[[5jrp]], [[Resolution|resolution]] 2.00Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[5jrp]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JRP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JRP FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5jrp]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5JRP OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5JRP FirstGlance]. <br> |
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jrp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jrp OCA], [http://pdbe.org/5jrp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jrp RCSB], [http://www.ebi.ac.uk/pdbsum/5jrp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jrp ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5jrp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5jrp OCA], [http://pdbe.org/5jrp PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5jrp RCSB], [http://www.ebi.ac.uk/pdbsum/5jrp PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5jrp ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | An atomic-detail model of the Marburg virus glycoprotein in complex with a neutralizing human monoclonal antibody designated MR78 was constructed using Phenix.Rosetta starting from a 3.6A crystallographic density map. The Asp at T6 in the HCDR3's bulged torso cannot form the canonical salt bridge as position T2 lacks an Arg or Lys residue. It instead engages in a hydrogen bond interaction with a Tyr contributed by the HCDR1 loop. This inter-CDR loop interaction stabilizes the bulged conformation needed for binding to the viral glycoprotein: a Tyr to Phe mutant displays a binding affinity reduced by a factor of at least 10. We found that 5% of a database of 465 million human antibody sequences has the same residues at T2 and T6 positions in HCDR3 and Tyr in HCDR1 that could potentially form this Asp-Tyr interaction, and that this interaction might contribute to a non-canonical bulged torso conformation. | ||
| + | |||
| + | Role of Non-local Interactions between CDR Loops in Binding Affinity of MR78 Antibody to Marburg Virus Glycoprotein.,Sangha AK, Dong J, Williamson L, Hashiguchi T, Saphire EO, Crowe JE Jr, Meiler J Structure. 2017 Dec 5;25(12):1820-1828.e2. doi: 10.1016/j.str.2017.10.005. Epub, 2017 Nov 16. PMID:29153506<ref>PMID:29153506</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5jrp" style="background-color:#fffaf0;"></div> | ||
| + | |||
| + | ==See Also== | ||
| + | *[[Monoclonal Antibodies 3D structures|Monoclonal Antibodies 3D structures]] | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Human]] | ||
[[Category: Crowe, J]] | [[Category: Crowe, J]] | ||
[[Category: Dong, J]] | [[Category: Dong, J]] | ||
Revision as of 05:48, 25 April 2018
crystal structure of monoclonal antibody MR78 Fab
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