6fas
From Proteopedia
(Difference between revisions)
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<StructureSection load='6fas' size='340' side='right' caption='[[6fas]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='6fas' size='340' side='right' caption='[[6fas]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
| - | <table><tr><td colspan='2'>[[6fas]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAS FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6fas]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FAS OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FAS FirstGlance]. <br> |
</td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr> | </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=CME:S,S-(2-HYDROXYETHYL)THIOCYSTEINE'>CME</scene></td></tr> | ||
| + | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">VAL1, HSI2, At2g30470, T6B20.17 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fas OCA], [http://pdbe.org/6fas PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fas RCSB], [http://www.ebi.ac.uk/pdbsum/6fas PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fas ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fas FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fas OCA], [http://pdbe.org/6fas PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fas RCSB], [http://www.ebi.ac.uk/pdbsum/6fas PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fas ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[[http://www.uniprot.org/uniprot/VAL1_ARATH VAL1_ARATH]] Transcriptional repressor of gene expression involved in embryonic pathways, such as LEC1, ABI3, and FUS3. Repressor of the sugar-inducible genes involved in the seed maturation program in seedlings. Plays an essential role in regulating the transition from seed maturation to seedling growth. Functionally redundant with VAL2/HSL1.<ref>PMID:15894743</ref> <ref>PMID:17158584</ref> <ref>PMID:17267611</ref> | [[http://www.uniprot.org/uniprot/VAL1_ARATH VAL1_ARATH]] Transcriptional repressor of gene expression involved in embryonic pathways, such as LEC1, ABI3, and FUS3. Repressor of the sugar-inducible genes involved in the seed maturation program in seedlings. Plays an essential role in regulating the transition from seed maturation to seedling growth. Functionally redundant with VAL2/HSL1.<ref>PMID:15894743</ref> <ref>PMID:17158584</ref> <ref>PMID:17267611</ref> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Arabidopsis thaliana requires a prolonged period of cold exposure during winter to initiate flowering in a process termed vernalization. Exposure to cold induces epigenetic silencing of the FLOWERING LOCUS C (FLC) gene by Polycomb group (PcG) proteins. A key role in this epigenetic switch is played by transcriptional repressors VAL1 and VAL2, which specifically recognize Sph/RY DNA sequences within FLC via B3 DNA binding domains, and mediate recruitment of PcG silencing machinery. To understand the structural mechanism of site-specific DNA recognition by VAL1, we have solved the crystal structure of VAL1 B3 domain (VAL1-B3) bound to a 12 bp oligoduplex containing the canonical Sph/RY DNA sequence 5'-CATGCA-3'/5'-TGCATG-3'. We find that VAL1-B3 makes H-bonds and van der Waals contacts to DNA bases of all six positions of the canonical Sph/RY element. In agreement with the structure, in vitro DNA binding studies show that VAL1-B3 does not tolerate substitutions at any position of the 5'-TGCATG-3' sequence. The VAL1-B3-DNA structure presented here provides a structural model for understanding the specificity of plant B3 domains interacting with the Sph/RY and other DNA sequences. | ||
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| + | Structural basis of DNA target recognition by the B3 domain of Arabidopsis epigenome reader VAL1.,Sasnauskas G, Kauneckaite K, Siksnys V Nucleic Acids Res. 2018 Apr 6. pii: 4962483. doi: 10.1093/nar/gky256. PMID:29660015<ref>PMID:29660015</ref> | ||
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| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 6fas" style="background-color:#fffaf0;"></div> | ||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Arath]] | ||
[[Category: Sasnauskas, G]] | [[Category: Sasnauskas, G]] | ||
[[Category: B3 dna binding domain]] | [[Category: B3 dna binding domain]] | ||
Revision as of 06:08, 25 April 2018
Crystal structure of VAL1 B3 domain in complex with cognate DNA
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