2hje
From Proteopedia
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|PDB= 2hje |SIZE=350|CAPTION= <scene name='initialview01'>2hje</scene>, resolution 1.70Å | |PDB= 2hje |SIZE=350|CAPTION= <scene name='initialview01'>2hje</scene>, resolution 1.70Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=NI:NICKEL (II) ION'>NI</scene> | + | |LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Histidine_kinase Histidine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.13.3 2.7.13.3] </span> |
|GENE= luxQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]) | |GENE= luxQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=669 Vibrio harveyi]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2hj9|2HJ9]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hje FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hje OCA], [http://www.ebi.ac.uk/pdbsum/2hje PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hje RCSB]</span> | ||
}} | }} | ||
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[[Category: Kelly, R C.]] | [[Category: Kelly, R C.]] | ||
[[Category: Neiditch, M B.]] | [[Category: Neiditch, M B.]] | ||
| - | [[Category: NI]] | ||
[[Category: autoinducer-2 (ai-2)]] | [[Category: autoinducer-2 (ai-2)]] | ||
[[Category: histidine sensor kinase]] | [[Category: histidine sensor kinase]] | ||
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[[Category: quorum sensing]] | [[Category: quorum sensing]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:31:17 2008'' |
Revision as of 00:31, 31 March 2008
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| , resolution 1.70Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | luxQ (Vibrio harveyi) | ||||||
| Activity: | Histidine kinase, with EC number 2.7.13.3 | ||||||
| Related: | 2HJ9
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Vibrio harveyi LuxQ periplasmic domain
Overview
Bacteria sense their environment using receptors of the histidine sensor kinase family, but how kinase activity is regulated by ligand binding is not well understood. Autoinducer-2 (AI-2), a secreted signaling molecule originally identified in studies of the marine bacterium Vibrio harveyi, regulates quorum-sensing responses and allows communication between different bacterial species. AI-2 signal transduction in V. harveyi requires the integral membrane receptor LuxPQ, comprised of periplasmic binding protein (LuxP) and histidine sensor kinase (LuxQ) subunits. Combined X-ray crystallographic and functional studies show that AI-2 binding causes a major conformational change within LuxP, which in turn stabilizes a quaternary arrangement in which two LuxPQ monomers are asymmetrically associated. We propose that formation of this asymmetric quaternary structure is responsible for repressing the kinase activity of both LuxQ subunits and triggering the transition of V. harveyi into quorum-sensing mode.
About this Structure
2HJE is a Single protein structure of sequence from Vibrio harveyi. Full crystallographic information is available from OCA.
Reference
Ligand-induced asymmetry in histidine sensor kinase complex regulates quorum sensing., Neiditch MB, Federle MJ, Pompeani AJ, Kelly RC, Swem DL, Jeffrey PD, Bassler BL, Hughson FM, Cell. 2006 Sep 22;126(6):1095-108. PMID:16990134
Page seeded by OCA on Mon Mar 31 03:31:17 2008
