2hki

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|PDB= 2hki |SIZE=350|CAPTION= <scene name='initialview01'>2hki</scene>, resolution 3.00&Aring;
|PDB= 2hki |SIZE=350|CAPTION= <scene name='initialview01'>2hki</scene>, resolution 3.00&Aring;
|SITE=
|SITE=
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|LIGAND= <scene name='pdbligand=SO4:SULFATE ION'>SO4</scene>
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|LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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|ACTIVITY= [http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+))_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.13 1.2.1.13]
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Glyceraldehyde-3-phosphate_dehydrogenase_(NADP(+))_(phosphorylating) Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.2.1.13 1.2.1.13] </span>
|GENE=
|GENE=
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|DOMAIN=
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hki FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hki OCA], [http://www.ebi.ac.uk/pdbsum/2hki PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hki RCSB]</span>
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[[Category: Spinacia oleracea]]
[[Category: Spinacia oleracea]]
[[Category: Camara-Artigas, A.]]
[[Category: Camara-Artigas, A.]]
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[[Category: SO4]]
 
[[Category: apo-form]]
[[Category: apo-form]]
[[Category: rossmann fold]]
[[Category: rossmann fold]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Thu Mar 20 17:18:09 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:31:40 2008''

Revision as of 00:31, 31 March 2008


PDB ID 2hki

Drag the structure with the mouse to rotate
, resolution 3.00Å
Ligands:
Activity: Glyceraldehyde-3-phosphate dehydrogenase (NADP(+)) (phosphorylating), with EC number 1.2.1.13
Resources: FirstGlance, OCA, PDBsum, RCSB
Coordinates: save as pdb, mmCIF, xml



Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform


Overview

Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 A and the other of which belonged to a new space group I4(1)22, with unit-cell parameters a = b = 120.9, c = 154.5 A. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 A. Differences at the NAD(+)/NADP(+)-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme.

About this Structure

2HKI is a Single protein structure of sequence from Spinacia oleracea. Full crystallographic information is available from OCA.

Reference

Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form., Camara-Artigas A, Hirasawa M, Knaff DB, Wang M, Allen JP, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Nov 1;62(Pt, 11):1087-92. Epub 2006 Oct 25. PMID:17077485

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