2hnk
From Proteopedia
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|PDB= 2hnk |SIZE=350|CAPTION= <scene name='initialview01'>2hnk</scene>, resolution 2.300Å | |PDB= 2hnk |SIZE=350|CAPTION= <scene name='initialview01'>2hnk</scene>, resolution 2.300Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hnk OCA], [http://www.ebi.ac.uk/pdbsum/2hnk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hnk RCSB]</span> | ||
}} | }} | ||
| Line 25: | Line 28: | ||
[[Category: Hou, X.]] | [[Category: Hou, X.]] | ||
[[Category: Wei, Z.]] | [[Category: Wei, Z.]] | ||
| - | [[Category: PEG]] | ||
| - | [[Category: SAH]] | ||
| - | [[Category: SO4]] | ||
[[Category: modified rossman fold]] | [[Category: modified rossman fold]] | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:32:44 2008'' |
Revision as of 00:32, 31 March 2008
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| , resolution 2.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans
Overview
The S-adenosylmethionine (SAM)-dependent O-methyltransferase from Leptospira interrogans (LiOMT) expressed by gene LA0415 belongs to the Methyltransf_3 family (Pfam PF01596). In this family all of the five bacterial homologues with known function are reported as SAM-dependent O-methylstransferases involved in antibiotic production. The crystal structure of LiOMT in complex with S-adenosylhomocysteine reported here is the first bacterial protein structure in this family. The LiOMT structure shows a conserved SAM-binding region and a probable metal-dependent catalytic site. The molecules of LiOMT generate homodimers by N-terminal swapping, which assists the pre-organization of the substrate-binding site. Based on the sequence and structural analysis, it is implied by the catalytic and substrate-binding site that the substrate of LiOMT is a phenolic derivative, which probably has a large ring-shaped moiety.
About this Structure
2HNK is a Single protein structure of sequence from Leptospira interrogans. Full crystallographic information is available from OCA.
Reference
Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans., Hou X, Wang Y, Zhou Z, Bao S, Lin Y, Gong W, J Struct Biol. 2007 Sep;159(3):523-8. Epub 2007 Apr 30. PMID:17561415
Page seeded by OCA on Mon Mar 31 03:32:44 2008
