2hrv
From Proteopedia
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|PDB= 2hrv |SIZE=350|CAPTION= <scene name='initialview01'>2hrv</scene>, resolution 1.95Å | |PDB= 2hrv |SIZE=350|CAPTION= <scene name='initialview01'>2hrv</scene>, resolution 1.95Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ZN:ZINC ION'>ZN</scene> | + | |LIGAND= <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Picornain_2A Picornain 2A], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.29 3.4.22.29] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hrv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hrv OCA], [http://www.ebi.ac.uk/pdbsum/2hrv PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hrv RCSB]</span> | ||
}} | }} | ||
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[[Category: Petersen, J F.W.]] | [[Category: Petersen, J F.W.]] | ||
[[Category: Skern, T.]] | [[Category: Skern, T.]] | ||
| - | [[Category: ZN]] | ||
[[Category: hydrolase (cysteine proteinase)]] | [[Category: hydrolase (cysteine proteinase)]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:34:34 2008'' |
Revision as of 00:34, 31 March 2008
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| , resolution 1.95Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Activity: | Picornain 2A, with EC number 3.4.22.29 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
2A CYSTEINE PROTEINASE FROM HUMAN RHINOVIRUS 2
Overview
The crystal structure of the 2A proteinase from human rhinovirus serotype 2 (HRV2-2A(pro)) has been solved to 1.95 A resolution. The structure has an unusual, although chymotrypsin-related, fold comprising a unique four-stranded beta sheet as the N-terminal domain and a six-stranded beta barrel as the C-terminal domain. A tightly bound zinc ion, essential for the stability of HRV2-2A(pro), is tetrahedrally coordinated by three cysteine sulfurs and one histidine nitrogen. The active site consists of a catalytic triad formed by His18, Asp35 and Cys106. Asp35 is additionally involved in an extensive hydrogen-bonding network. Modelling studies reveal a substrate-induced fit that explains the specificity of the subsites S4, S2, S1 and S1'. The structure of HRV2-2A(pro) suggests the mechanism of the cis cleavage and its release from the polyprotein.
About this Structure
2HRV is a Single protein structure of sequence from Human rhinovirus 2. Full crystallographic information is available from OCA.
Reference
The structure of the 2A proteinase from a common cold virus: a proteinase responsible for the shut-off of host-cell protein synthesis., Petersen JF, Cherney MM, Liebig HD, Skern T, Kuechler E, James MN, EMBO J. 1999 Oct 15;18(20):5463-75. PMID:10523291
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