2hvk
From Proteopedia
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|PDB= 2hvk |SIZE=350|CAPTION= <scene name='initialview01'>2hvk</scene>, resolution 1.900Å | |PDB= 2hvk |SIZE=350|CAPTION= <scene name='initialview01'>2hvk</scene>, resolution 1.900Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=L2C:(2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL+LAURATE'>L2C</scene>, | + | |LIGAND= <scene name='pdbligand=F09:NONAN-1-OL'>F09</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=L2C:(2S)-3-HYDROXY-2-(NONANOYLOXY)PROPYL+LAURATE'>L2C</scene>, <scene name='pdbligand=TBA:TETRABUTYLAMMONIUM+ION'>TBA</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= kcsA, skc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1916 Streptomyces lividans]) | |GENE= kcsA, skc1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1916 Streptomyces lividans]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1k4c|1K4C]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hvk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hvk OCA], [http://www.ebi.ac.uk/pdbsum/2hvk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hvk RCSB]</span> | ||
}} | }} | ||
| Line 24: | Line 27: | ||
[[Category: Streptomyces lividans]] | [[Category: Streptomyces lividans]] | ||
[[Category: Zhou, Y.]] | [[Category: Zhou, Y.]] | ||
| - | [[Category: F09]] | ||
| - | [[Category: K]] | ||
| - | [[Category: L2C]] | ||
| - | [[Category: TBA]] | ||
[[Category: k+]] | [[Category: k+]] | ||
[[Category: kcsa]] | [[Category: kcsa]] | ||
| Line 34: | Line 33: | ||
[[Category: tetrabutylammonium]] | [[Category: tetrabutylammonium]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:35:58 2008'' |
Revision as of 00:35, 31 March 2008
| |||||||
| , resolution 1.900Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , , , | ||||||
| Gene: | kcsA, skc1 (Streptomyces lividans) | ||||||
| Related: | 1K4C
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
crystal structure of the KcsA-Fab-TBA complex in high K+
Overview
K(+) channels play essential roles in regulating membrane excitability of many diverse cell types by selectively conducting K(+) ions through their pores. Many diverse molecules can plug the pore and modulate the K(+) current. Quaternary ammonium (QA) ions are a class of pore blockers that have been used for decades by biophysicists to probe the pore, leading to important insights into the structure-function relation of K(+) channels. However, many key aspects of the QA-blocking mechanisms remain unclear to date, and understanding these questions requires high resolution structural information. Here, we address the question of whether intracellular QA blockade causes conformational changes of the K(+) channel selectivity filter. We have solved the structures of the KcsA K(+) channel in complex with tetrabutylammonium (TBA) and tetrabutylantimony (TBSb) under various ionic conditions. Our results demonstrate that binding of TBA or TBSb causes no significant change in the KcsA structure at high concentrations of permeant ions. We did observe the expected conformational change of the filter at low concentration of K(+), but this change appears to be independent of TBA or TBSb blockade.
About this Structure
2HVK is a Single protein structure of sequence from Mus musculus and Streptomyces lividans. Full crystallographic information is available from OCA.
Reference
Crystallographic study of the tetrabutylammonium block to the KcsA K+ channel., Yohannan S, Hu Y, Zhou Y, J Mol Biol. 2007 Feb 23;366(3):806-14. Epub 2006 Dec 2. PMID:17196615
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