2hzc
From Proteopedia
| Line 4: | Line 4: | ||
|PDB= 2hzc |SIZE=350|CAPTION= <scene name='initialview01'>2hzc</scene>, resolution 1.47Å | |PDB= 2hzc |SIZE=350|CAPTION= <scene name='initialview01'>2hzc</scene>, resolution 1.47Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= U2AF2, U2AF65 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= U2AF2, U2AF65 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2g4b|2G4B]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hzc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hzc OCA], [http://www.ebi.ac.uk/pdbsum/2hzc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hzc RCSB]</span> | ||
}} | }} | ||
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[[Category: Sickmier, E A.]] | [[Category: Sickmier, E A.]] | ||
[[Category: Thickman, K R.]] | [[Category: Thickman, K R.]] | ||
| - | [[Category: P6G]] | ||
| - | [[Category: ZN]] | ||
[[Category: alternative conformation]] | [[Category: alternative conformation]] | ||
[[Category: rna recognition]] | [[Category: rna recognition]] | ||
| Line 32: | Line 33: | ||
[[Category: rrm]] | [[Category: rrm]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:37:34 2008'' |
Revision as of 00:37, 31 March 2008
| |||||||
| , resolution 1.47Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | U2AF2, U2AF65 (Homo sapiens) | ||||||
| Related: | 2G4B
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of the N-terminal RRM of the U2AF large subunit
Overview
The essential pre-mRNA splicing factor, U2 auxiliary factor 65KD (U2AF(65)) recognizes the polypyrimidine tract (Py-tract) consensus sequence of the pre-mRNA using two RNA recognition motifs (RRMs), the most prevalent class of eukaryotic RNA-binding domain. The Py-tracts of higher eukaryotic pre-mRNAs are often interrupted with purines, yet U2AF(65) must identify these degenerate Py-tracts for accurate pre-mRNA splicing. Previously, the structure of a U2AF(65) variant in complex with poly(U) RNA suggested that rearrangement of flexible side-chains or bound water molecules may contribute to degenerate Py-tract recognition by U2AF(65). Here, the X-ray structure of the N-terminal RRM domain of U2AF(65) (RRM1) is described at 1.47 A resolution in the absence of RNA. Notably, RNA-binding by U2AF(65) selectively stabilizes pre-existing alternative conformations of three side-chains located at the RNA interface (Arg150, Lys225, and Arg227). Additionally, a flexible loop connecting the beta2/beta3 strands undergoes a conformational change to interact with the RNA. These pre-existing alternative conformations may contribute to the ability of U2AF(65) to recognize a variety of Py-tract sequences. This rare, high-resolution view of an important member of the RRM class of RNA-binding domains highlights the role of alternative side-chain conformations in RNA recognition.
About this Structure
2HZC is a Single protein structure of sequence from Homo sapiens. This structure supersedes the now removed PDB entry 2FZR. Full crystallographic information is available from OCA.
Reference
Alternative conformations at the RNA-binding surface of the N-terminal U2AF(65) RNA recognition motif., Thickman KR, Sickmier EA, Kielkopf CL, J Mol Biol. 2007 Feb 23;366(3):703-10. Epub 2006 Dec 2. PMID:17188295
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