2hzm
From Proteopedia
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|PDB= 2hzm |SIZE=350|CAPTION= <scene name='initialview01'>2hzm</scene>, resolution 2.4Å | |PDB= 2hzm |SIZE=350|CAPTION= <scene name='initialview01'>2hzm</scene>, resolution 2.4Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE ION'>PO4</scene> | + | |LIGAND= <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= SRB2, HRS2, MED20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB5, MED18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | |GENE= SRB2, HRS2, MED20 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]), SRB5, MED18 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=4932 Saccharomyces cerevisiae]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1zp2|1ZP2]], [[1yke|1YKE]], [[1ykh|1YKH]], [[2hzs|2HZS]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hzm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hzm OCA], [http://www.ebi.ac.uk/pdbsum/2hzm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hzm RCSB]</span> | ||
}} | }} | ||
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[[Category: Rother, S.]] | [[Category: Rother, S.]] | ||
[[Category: Straesser, K.]] | [[Category: Straesser, K.]] | ||
| - | [[Category: PO4]] | ||
[[Category: beta barrel]] | [[Category: beta barrel]] | ||
[[Category: channel]] | [[Category: channel]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:37:41 2008'' |
Revision as of 00:37, 31 March 2008
| |||||||
| , resolution 2.4Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | SRB2, HRS2, MED20 (Saccharomyces cerevisiae), SRB5, MED18 (Saccharomyces cerevisiae) | ||||||
| Related: | 1ZP2, 1YKE, 1YKH, 2HZS
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of the Mediator head subcomplex Med18/20
Overview
The Mediator head module stimulates basal RNA polymerase II (Pol II) transcription and enables transcriptional regulation. Here we show that the head subunits Med8, Med18 and Med20 form a subcomplex (Med8/18/20) with two submodules. The highly conserved N-terminal domain of Med8 forms one submodule that binds the TATA box-binding protein (TBP) in vitro and is essential in vivo. The second submodule consists of the C-terminal region of Med8 (Med8C), Med18 and Med20. X-ray analysis of this submodule reveals that Med18 and Med20 form related beta-barrel folds. A conserved putative protein-interaction face on the Med8C/18/20 submodule includes sites altered by srb mutations, which counteract defects resulting from Pol II truncation. Our results and published data support a positive role of the Med8/18/20 subcomplex in initiation-complex formation and suggest that the Mediator head contains a multipartite TBP-binding site that can be modulated by transcriptional activators.
About this Structure
2HZM is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structure and TBP binding of the Mediator head subcomplex Med8-Med18-Med20., Lariviere L, Geiger S, Hoeppner S, Rother S, Strasser K, Cramer P, Nat Struct Mol Biol. 2006 Oct;13(10):895-901. Epub 2006 Sep 10. PMID:16964259
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