2i42
From Proteopedia
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|SITE= | |SITE= | ||
|LIGAND= <scene name='pdbligand=I42:HYDROXY(DIOXIDO)OXOVANADIUM'>I42</scene> | |LIGAND= <scene name='pdbligand=I42:HYDROXY(DIOXIDO)OXOVANADIUM'>I42</scene> | ||
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Protein-tyrosine-phosphatase Protein-tyrosine-phosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.48 3.1.3.48] </span> |
|GENE= yopH, yop51 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 Yersinia enterocolitica]) | |GENE= yopH, yop51 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=630 Yersinia enterocolitica]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1yts|1YTS]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i42 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i42 OCA], [http://www.ebi.ac.uk/pdbsum/2i42 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i42 RCSB]</span> | ||
}} | }} | ||
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[[Category: Saper, M A.]] | [[Category: Saper, M A.]] | ||
[[Category: Vijayalakshmi, J.]] | [[Category: Vijayalakshmi, J.]] | ||
| - | [[Category: I42]] | ||
[[Category: crystal structure]] | [[Category: crystal structure]] | ||
[[Category: transition state analogue]] | [[Category: transition state analogue]] | ||
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[[Category: yersinia ptpase]] | [[Category: yersinia ptpase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:39:23 2008'' |
Revision as of 00:39, 31 March 2008
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| , resolution 2.2Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | |||||||
| Gene: | yopH, yop51 (Yersinia enterocolitica) | ||||||
| Activity: | Protein-tyrosine-phosphatase, with EC number 3.1.3.48 | ||||||
| Related: | 1YTS
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal structure of Yersinia protein tyrosine phosphatase complexed with vanadate, a transition state analogue
Overview
Engineering site-specific amino acid substitutions into the protein-tyrosine phosphatase (PTPase) PTP1 and the dual-specific vaccinia H1-related phosphatase (VHR), has kinetically isolated the two chemical steps of the reaction and provided a rare opportunity for examining transition states and directly observing the phosphoenzyme intermediate. Changing serine to alanine in the active-site sequence motif HCXXGXXRS shifted the rate-limiting step from intermediate formation to intermediate hydrolysis. Using phosphorus 31P NMR, the covalent thiol-phosphate intermediate was directly observed during catalytic turnover. The importance of the conserved aspartic acid (D92 in VHR and D181 in PTP1) in both chemical steps was established. Kinetic analysis of D92N and D181N mutants indicated that aspartic acid acts as a general acid by protonating the leaving-group phenolic oxygen. Structure-reactivity experiments with native and aspartate mutant enzymes established that proton transfer is concomitant with P-O cleavage, such that no charge develops on the phenolic oxygen. Steady- and presteady-state kinetics, as well as NMR analysis of the double mutant D92N/S131A (VHR), suggested that the conserved aspartic acid functions as a general base during intermediate hydrolysis. As a general base, aspartate would activate a water molecule to facilitate nucleophilic attack. The amino acids involved in transition-state stabilization for cysteinylphosphate hydrolysis were confirmed by the x-ray structure of the Yersinia PTPase complexed with vanadate, a transition-state mimic that binds covalently to the active-site cysteine. Consistent with the NMR, x-ray, biochemical, and kinetic data, a unifying mechanism for catalysis is proposed.
About this Structure
2I42 is a Single protein structure of sequence from Yersinia enterocolitica. Full crystallographic information is available from OCA.
Reference
Visualization of intermediate and transition-state structures in protein-tyrosine phosphatase catalysis., Denu JM, Lohse DL, Vijayalakshmi J, Saper MA, Dixon JE, Proc Natl Acad Sci U S A. 1996 Mar 19;93(6):2493-8. PMID:8637902
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