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6clh
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==1.37 A MicroED structure of GSNQNNF at 2.9 e- / A^2== | |
| + | <StructureSection load='6clh' size='340' side='right' caption='[[6clh]], [[Resolution|resolution]] 1.37Å' scene=''> | ||
| + | == Structural highlights == | ||
| + | <table><tr><td colspan='2'>[[6clh]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CLH OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CLH FirstGlance]. <br> | ||
| + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6clh FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6clh OCA], [http://pdbe.org/6clh PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6clh RCSB], [http://www.ebi.ac.uk/pdbsum/6clh PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6clh ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Micro-crystal electron diffraction (MicroED) combines the efficiency of electron scattering with diffraction to allow structure determination from nano-sized crystalline samples in cryoelectron microscopy (cryo-EM). It has been used to solve structures of a diverse set of biomolecules and materials, in some cases to sub-atomic resolution. However, little is known about the damaging effects of the electron beam on samples during such measurements. We assess global and site-specific damage from electron radiation on nanocrystals of proteinase K and of a prion hepta-peptide and find that the dynamics of electron-induced damage follow well-established trends observed in X-ray crystallography. Metal ions are perturbed, disulfide bonds are broken, and acidic side chains are decarboxylated while the diffracted intensities decay exponentially with increasing exposure. A better understanding of radiation damage in MicroED improves our assessment and processing of all types of cryo-EM data. | ||
| - | + | Analysis of Global and Site-Specific Radiation Damage in Cryo-EM.,Hattne J, Shi D, Glynn C, Zee CT, Gallagher-Jones M, Martynowycz MW, Rodriguez JA, Gonen T Structure. 2018 May 1;26(5):759-766.e4. doi: 10.1016/j.str.2018.03.021. Epub 2018, Apr 26. PMID:29706530<ref>PMID:29706530</ref> | |
| - | + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |
| - | [[Category: | + | </div> |
| + | <div class="pdbe-citations 6clh" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: Gallagher-Jones, M]] | ||
| + | [[Category: Glynn, C]] | ||
| + | [[Category: Gonen, T]] | ||
| + | [[Category: Hattne, J]] | ||
| + | [[Category: Martynowycz, M W]] | ||
| + | [[Category: Rodriguez, J A]] | ||
| + | [[Category: Shi, D]] | ||
| + | [[Category: Zee, C T]] | ||
| + | [[Category: Amyloid fibril]] | ||
| + | [[Category: Prion]] | ||
| + | [[Category: Protein fibril]] | ||
| + | [[Category: Zinc binding]] | ||
Revision as of 05:32, 16 May 2018
1.37 A MicroED structure of GSNQNNF at 2.9 e- / A^2
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