2i7t
From Proteopedia
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|PDB= 2i7t |SIZE=350|CAPTION= <scene name='initialview01'>2i7t</scene>, resolution 2.10Å | |PDB= 2i7t |SIZE=350|CAPTION= <scene name='initialview01'>2i7t</scene>, resolution 2.10Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand= | + | |LIGAND= <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= CPSF3, CPSF73 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | |GENE= CPSF3, CPSF73 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[2i7v|2I7V]], [[2i7x|2I7X]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i7t FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i7t OCA], [http://www.ebi.ac.uk/pdbsum/2i7t PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i7t RCSB]</span> | ||
}} | }} | ||
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[[Category: Tong, L.]] | [[Category: Tong, L.]] | ||
[[Category: Zhang, H.]] | [[Category: Zhang, H.]] | ||
| - | [[Category: SO4]] | ||
| - | [[Category: ZN]] | ||
[[Category: artemi]] | [[Category: artemi]] | ||
[[Category: double-strand break repair]] | [[Category: double-strand break repair]] | ||
| Line 34: | Line 35: | ||
[[Category: v(d)j recombination]] | [[Category: v(d)j recombination]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:40:55 2008'' |
Revision as of 00:40, 31 March 2008
| |||||||
| , resolution 2.10Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | CPSF3, CPSF73 (Homo sapiens) | ||||||
| Related: | 2I7V, 2I7X
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Structure of human CPSF-73
Overview
Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3'-end. Despite the characterization of many proteins that are required for the cleavage reaction, the identity of the endonuclease is not known. Recent analyses indicated that the 73-kDa subunit of cleavage and polyadenylation specificity factor (CPSF-73) might be the endonuclease for this and related reactions, although no direct data confirmed this. Here we report the crystal structures of human CPSF-73 at 2.1 A resolution, complexed with zinc ions and a sulphate that might mimic the phosphate group of the substrate, and the related yeast protein CPSF-100 (Ydh1) at 2.5 A resolution. Both CPSF-73 and CPSF-100 contain two domains, a metallo-beta-lactamase domain and a novel beta-CASP (named for metallo-beta-lactamase, CPSF, Artemis, Snm1, Pso2) domain. The active site of CPSF-73, with two zinc ions, is located at the interface of the two domains. Purified recombinant CPSF-73 possesses RNA endonuclease activity, and mutations that disrupt zinc binding in the active site abolish this activity. Our studies provide the first direct experimental evidence that CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.
About this Structure
2I7T is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:17128255
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