2bob

From Proteopedia

(Difference between revisions)

Revision as of 06:12, 16 May 2018

Potassium channel KcsA-Fab complex in thallium with tetrabutylammonium (TBA)

Structural highlights

2bob is a 3 chain structure with sequence from "actinomyces_lividans"_krasil'nikov_et_al._1965 "actinomyces lividans" krasil'nikov et al. 1965 and Mus musculus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, ,
Related:	1bl8, 1f6g, 1j95, 1jq1, 1jq2, 1jvm, 1k4c, 1k4d, 1r3i, 1r3j, 1r3k, 1r3l, 1s5h
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KCSA_STRLI] Acts as a pH-gated potassium ion channel; changing the cytosolic pH from 7 to 4 opens the channel, although it is not clear if this is the physiological stimulus for channel opening. Monovalent cation preference is K(+) > Rb(+) > NH4(+) >> Na(+) > Li(+).^[1]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Potassium channels catalyze the selective transfer of potassium across the cell membrane and are essential for setting the resting potential in cells, controlling heart rate and modulating the firing pattern in neurons. Tetraethylammonium (TEA) blocks ion conduction through potassium channels in a voltage-dependent manner from both sides of the membrane. Here we show the structural basis of TEA blockade by cocrystallizing the prokaryotic potassium channel KcsA with two selective TEA analogs. TEA binding at both sites alters ion occupancy in the selectivity filter; these findings underlie the mutual destabilization and voltage-dependence of TEA blockade. We propose that TEA blocks potassium channels by acting as a potassium analog at the dehydration transition step during permeation.

Structural basis of TEA blockade in a model potassium channel.,Lenaeus MJ, Vamvouka M, Focia PJ, Gross A Nat Struct Mol Biol. 2005 May;12(5):454-9. Epub 2005 Apr 24. PMID:15852022^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Schrempf H, Schmidt O, Kummerlen R, Hinnah S, Muller D, Betzler M, Steinkamp T, Wagner R. A prokaryotic potassium ion channel with two predicted transmembrane segments from Streptomyces lividans. EMBO J. 1995 Nov 1;14(21):5170-8. PMID:7489706
↑ Lenaeus MJ, Vamvouka M, Focia PJ, Gross A. Structural basis of TEA blockade in a model potassium channel. Nat Struct Mol Biol. 2005 May;12(5):454-9. Epub 2005 Apr 24. PMID:15852022 doi:10.1038/nsmb929

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bob"

@@ Line 1: / Line 1: @@
-==POTASSIUM CHANNEL KCSA-FAB COMPLEX IN THALLIUM WITH TETRABUTYLAMMONIUM (TBA)==
+==Potassium channel KcsA-Fab complex in thallium with tetrabutylammonium (TBA)==
 <StructureSection load='2bob' size='340' side='right' caption='[[2bob]], [[Resolution|resolution]] 2.76&Aring;' scene=''>
 == Structural highlights ==
@@ Line 5: / Line 6: @@
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene>, <scene name='pdbligand=TBA:TETRABUTYLAMMONIUM+ION'>TBA</scene>, <scene name='pdbligand=TL:THALLIUM+(I)+ION'>TL</scene></td></tr>
 <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bl8|1bl8]], [[1f6g|1f6g]], [[1j95|1j95]], [[1jq1|1jq1]], [[1jq2|1jq2]], [[1jvm|1jvm]], [[1k4c|1k4c]], [[1k4d|1k4d]], [[1r3i|1r3i]], [[1r3j|1r3j]], [[1r3k|1r3k]], [[1r3l|1r3l]], [[1s5h|1s5h]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bob OCA], [http://pdbe.org/2bob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bob RCSB], [http://www.ebi.ac.uk/pdbsum/2bob PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bob FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bob OCA], [http://pdbe.org/2bob PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bob RCSB], [http://www.ebi.ac.uk/pdbsum/2bob PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bob ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 13: / Line 14: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/2bob_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bo/2bob_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 30: / Line 31: @@
 ==See Also==
-*[[Antibody|Antibody]]
+*[[Antibody 3D structures|Antibody 3D structures]]
 *[[Potassium Channel|Potassium Channel]]
 == References ==

2bob

From Proteopedia

Revision as of 06:12, 16 May 2018

Potassium channel KcsA-Fab complex in thallium with tetrabutylammonium (TBA)

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox