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2bjq

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Revision as of 06:20, 16 May 2018

Crystal structure of the nematode sperm cell motility protein MFP2

Structural highlights

2bjq is a 1 chain structure with sequence from Ascsu. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	1bjr
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

The simplicity and specialization of the cell motility machinery of Ascaris sperm provides a powerful system in which to probe the basic molecular mechanism of amoeboid cell motility. Although Ascaris sperm locomotion closely resembles that seen in many other types of crawling cell, movement is generated by modulation of a cytoskeleton based on the major sperm protein (MSP) rather than the actin present in other cell types. The Ascaris motility machinery can be studied conveniently in a cell-free in vitro system based on the movement of plasma membrane vesicles by fibres constructed from bundles of MSP filaments. In addition to ATP, MSP and a plasma membrane protein, reconstitution of MSP motility in this cell-free extract requires cytosolic proteins to orchestrate the site-specific assembly and bundling of MSP filaments that generates locomotion. One of these proteins, MFP2, accelerates the rate of movement in this assay. Here, we describe crystal structures of two isoforms of MFP2 and show that both are constructed from two domains that have the same fold based on a novel, compact beta sheet arrangement. Patterns of conservation observed in a structure-based analysis of MFP2 sequences from different nematode species identified regions that may be putative functional interfaces involved both in interactions between MFP2 domains and also with other components of the sperm motility machinery. Analysis of the growth of fibres in vitro in the presence of added MFP2 indicated that MFP2 increases the rate of locomotion by enhancing the effective rate of MSP filament polymerization. This observation, together with the structural data, suggests that MFP2 may function in a manner analogous to formins in actin-based motility.

Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility.,Grant RP, Buttery SM, Ekman GC, Roberts TM, Stewart M J Mol Biol. 2005 Apr 1;347(3):583-95. PMID:15755452^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Grant RP, Buttery SM, Ekman GC, Roberts TM, Stewart M. Structure of MFP2 and its function in enhancing MSP polymerization in Ascaris sperm amoeboid motility. J Mol Biol. 2005 Apr 1;347(3):583-95. PMID:15755452 doi:http://dx.doi.org/10.1016/j.jmb.2005.01.054

1 Structural highlights
2 Evolutionary Conservation
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2bjq"

@@ Line 1: / Line 1: @@
-==CRYSTAL STRUCTURE OF THE NEMATODE SPERM CELL MOTILITY PROTEIN MFP2==
+==Crystal structure of the nematode sperm cell motility protein MFP2==
 <StructureSection load='2bjq' size='340' side='right' caption='[[2bjq]], [[Resolution|resolution]] 1.75&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2bjq]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Ascsu Ascsu]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2BJQ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2BJQ FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1bjr|1bjr]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjq OCA], [http://pdbe.org/2bjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bjq RCSB], [http://www.ebi.ac.uk/pdbsum/2bjq PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2bjq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2bjq OCA], [http://pdbe.org/2bjq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2bjq RCSB], [http://www.ebi.ac.uk/pdbsum/2bjq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2bjq ProSAT]</span></td></tr>
 </table>
 == Evolutionary Conservation ==
@@ Line 10: / Line 11: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjq_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/bj/2bjq_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>

2bjq

From Proteopedia

Revision as of 06:20, 16 May 2018

Crystal structure of the nematode sperm cell motility protein MFP2

Structural highlights

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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