2b2e

From Proteopedia

(Difference between revisions)

Revision as of 06:35, 16 May 2018

RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid

Structural highlights

2b2e is a 5 chain structure with sequence from Bacteriophage ms2. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2bny, 1aq3, 1aq4, 1bms, 1dzs, 1e6t, 2b2d, 2b2g
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[COAT_BPMS2] Forms the phage shell; binds to the phage RNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Sequence-specific interactions between RNA stem-loops and coat protein (CP) subunits play vital roles in the life cycles of the RNA bacteriophages, e.g., by allowing translational repression of their replicase cistrons and tagging their own RNA genomes for encapsidation. The CPs of bacteriophages Qbeta and MS2 each discriminate in favor of their cognate translational operators, even in the presence of closely related operators from other phages in vivo. Discrete mutations within the MS2 CP have been shown to relax this discrimination in vitro. We have determined the structures of eight complexes between such mutants and both MS2 and Qbeta stem-loops with X-ray crystallography. In conjunction with previously determined in vivo repression data, the structures enable us to propose the molecular basis for the discrimination mechanism.

Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2.,Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG Structure. 2006 Mar;14(3):487-95. PMID:16531233^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Horn WT, Tars K, Grahn E, Helgstrand C, Baron AJ, Lago H, Adams CJ, Peabody DS, Phillips SE, Stonehouse NJ, Liljas L, Stockley PG. Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2. Structure. 2006 Mar;14(3):487-95. PMID:16531233 doi:http://dx.doi.org/10.1016/j.str.2005.12.006

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2b2e"

@@ Line 1: / Line 1: @@
 ==RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid==
 <StructureSection load='2b2e' size='340' side='right' caption='[[2b2e]], [[Resolution|resolution]] 3.15&Aring;' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2b2e]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_ms2 Bacteriophage ms2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2B2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2B2E FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2bny|2bny]], [[1aq3|1aq3]], [[1aq4|1aq4]], [[1bms|1bms]], [[1dzs|1dzs]], [[1e6t|1e6t]], [[2b2d|2b2d]], [[2b2g|2b2g]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2e OCA], [http://pdbe.org/2b2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b2e RCSB], [http://www.ebi.ac.uk/pdbsum/2b2e PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2b2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2b2e OCA], [http://pdbe.org/2b2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2b2e RCSB], [http://www.ebi.ac.uk/pdbsum/2b2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2b2e ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 12: / Line 13: @@
 Check<jmol>
   <jmolCheckbox>
-    <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2e_consurf.spt"</scriptWhenChecked>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/b2/2b2e_consurf.spt"</scriptWhenChecked>
     <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
     <text>to colour the structure by Evolutionary Conservation</text>
   </jmolCheckbox>
-</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/chain_selection.php?pdb_ID=2ata ConSurf].
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2b2e ConSurf].
 <div style="clear:both"></div>
 <div style="background-color:#fffaf0;">

2b2e

From Proteopedia

Revision as of 06:35, 16 May 2018

RNA stemloop from bacteriophage MS2 complexed with an N87S,E89K mutant MS2 capsid

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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