2ibp
From Proteopedia
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|PDB= 2ibp |SIZE=350|CAPTION= <scene name='initialview01'>2ibp</scene>, resolution 1.60Å | |PDB= 2ibp |SIZE=350|CAPTION= <scene name='initialview01'>2ibp</scene>, resolution 1.60Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene> | + | |LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Citrate_(Si)-synthase Citrate (Si)-synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.3.3.1 2.3.3.1] </span> |
|GENE= PAE1689 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13773 Pyrobaculum aerophilum]) | |GENE= PAE1689 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=13773 Pyrobaculum aerophilum]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ibp FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ibp OCA], [http://www.ebi.ac.uk/pdbsum/2ibp PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ibp RCSB]</span> | ||
}} | }} | ||
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[[Category: Cascio, D.]] | [[Category: Cascio, D.]] | ||
[[Category: Yeates, T O.]] | [[Category: Yeates, T O.]] | ||
| - | [[Category: ACT]] | ||
| - | [[Category: MG]] | ||
[[Category: catenane]] | [[Category: catenane]] | ||
[[Category: citrate synthase]] | [[Category: citrate synthase]] | ||
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[[Category: thermophilic]] | [[Category: thermophilic]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:42:13 2008'' |
Revision as of 00:42, 31 March 2008
| |||||||
| , resolution 1.60Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | PAE1689 (Pyrobaculum aerophilum) | ||||||
| Activity: | Citrate (Si)-synthase, with EC number 2.3.3.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of Citrate Synthase from Pyrobaculum aerophilum
Overview
A growing number of organisms have been discovered inhabiting extreme environments, including temperatures in excess of 100 degrees C. How cellular proteins from such organisms retain their native folds under extreme conditions is still not fully understood. Recent computational and structural studies have identified disulfide bonding as an important mechanism for stabilizing intracellular proteins in certain thermophilic microbes. Here, we present the first proteomic analysis of intracellular disulfide bonding in the hyperthermophilic archaeon Pyrobaculum aerophilum. Our study reveals that the utilization of disulfide bonds extends beyond individual proteins to include many protein-protein complexes. We report the 1.6 A crystal structure of one such complex, a citrate synthase homodimer. The structure contains two intramolecular disulfide bonds, one per subunit, which result in the cyclization of each protein chain in such a way that the two chains are topologically interlinked, rendering them inseparable. This unusual feature emphasizes the variety and sophistication of the molecular mechanisms that can be achieved by evolution.
About this Structure
2IBP is a Single protein structure of sequence from Pyrobaculum aerophilum. Full crystallographic information is available from OCA.
Reference
Discovery of a thermophilic protein complex stabilized by topologically interlinked chains., Boutz DR, Cascio D, Whitelegge J, Perry LJ, Yeates TO, J Mol Biol. 2007 May 18;368(5):1332-44. Epub 2007 Mar 6. PMID:17395198
Page seeded by OCA on Mon Mar 31 03:42:13 2008
