2ipk
From Proteopedia
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|PDB= 2ipk |SIZE=350|CAPTION= <scene name='initialview01'>2ipk</scene>, resolution 2.300Å | |PDB= 2ipk |SIZE=350|CAPTION= <scene name='initialview01'>2ipk</scene>, resolution 2.300Å | ||
|SITE= | |SITE= | ||
| - | |LIGAND= | + | |LIGAND= <scene name='pdbligand=4DP:3-[5-(DIMETHYLAMINO)-1,3-DIOXO-1,3-DIHYDRO-2H-ISOINDOL-2-YL]-L-ALANINE'>4DP</scene>, <scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene> |
|ACTIVITY= | |ACTIVITY= | ||
|GENE= HLA-DRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), HLA-DRB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), entC3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | |GENE= HLA-DRA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), HLA-DRB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), entC3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 Staphylococcus aureus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1jwu|1JWU]], [[1dlh|1DLH]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ipk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ipk OCA], [http://www.ebi.ac.uk/pdbsum/2ipk PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ipk RCSB]</span> | ||
}} | }} | ||
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[[Category: Nguyen, T T.]] | [[Category: Nguyen, T T.]] | ||
[[Category: Stern, L J.]] | [[Category: Stern, L J.]] | ||
| - | [[Category: 4-(n]] | + | [[Category: 4-(n,n-dimethylamino)phthalimidoalanyl]] |
[[Category: dapa]] | [[Category: dapa]] | ||
[[Category: dr1]] | [[Category: dr1]] | ||
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[[Category: major histocompatibility complex]] | [[Category: major histocompatibility complex]] | ||
[[Category: mhc]] | [[Category: mhc]] | ||
| - | [[Category: n-dimethylamino)phthalimidoalanyl]] | ||
[[Category: sec3]] | [[Category: sec3]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:47:13 2008'' |
Revision as of 00:47, 31 March 2008
| |||||||
| , resolution 2.300Å | |||||||
|---|---|---|---|---|---|---|---|
| Ligands: | , | ||||||
| Gene: | HLA-DRA (Homo sapiens), HLA-DRB1 (Homo sapiens), entC3 (Staphylococcus aureus) | ||||||
| Related: | 1JWU, 1DLH
| ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Crystal Structure of the MHC Class II Molecule HLA-DR1 in Complex with the Fluorogenic Peptide, AcPKXVKQNTLKLAT (X=3-[5-(dimethylamino)-1,3-dioxo-1,3-dihydro-2H-isoindol-2-yl]-L-alanine) and the Superantigen, SEC3 Variant 3B2
Overview
A crucial step in the immune response is the binding of antigenic peptides to major histocompatibility complex (MHC) proteins. Class II MHC proteins present their bound peptides to CD4(+) T cells, thereby helping to activate both the humoral and the cellular arms of the adaptive immune response. Peptide loading onto class II MHC proteins is regulated temporally, spatially and developmentally in antigen-presenting cells. To help visualize these processes, we have developed a series of novel fluorogenic probes that incorporate the environment-sensitive amino acid analogs 6-N,N-dimethylamino-2-3-naphthalimidoalanine and 4-N,N-dimethylaminophthalimidoalanine. Upon binding to class II MHC proteins these fluorophores show large changes in emission spectra, quantum yield and fluorescence lifetime. Peptides incorporating these fluorophores bind specifically to class II MHC proteins on antigen-presenting cells and can be used to follow peptide binding in vivo. Using these probes we have tracked a developmentally regulated cell-surface peptide-binding activity in primary human monocyte-derived dendritic cells.
About this Structure
2IPK is a Protein complex structure of sequences from Homo sapiens and Staphylococcus aureus. Full crystallographic information is available from OCA.
Reference
Fluorogenic probes for monitoring peptide binding to class II MHC proteins in living cells., Venkatraman P, Nguyen TT, Sainlos M, Bilsel O, Chitta S, Imperiali B, Stern LJ, Nat Chem Biol. 2007 Apr;3(4):222-8. Epub 2007 Mar 11. PMID:17351628
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