6cq2
From Proteopedia
(Difference between revisions)
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| - | '''Unreleased structure''' | ||
| - | + | ==Crystal structure of Mycobacterium tuberculosis Topoisomerase I in complex with oligonucleotide MTS2-12 and Magnesium== | |
| - | + | <StructureSection load='6cq2' size='340' side='right' caption='[[6cq2]], [[Resolution|resolution]] 3.00Å' scene=''> | |
| - | + | == Structural highlights == | |
| - | + | <table><tr><td colspan='2'>[[6cq2]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CQ2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CQ2 FirstGlance]. <br> | |
| - | + | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |
| - | [[Category: | + | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5d5h|5d5h]]</td></tr> |
| + | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_topoisomerase DNA topoisomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.99.1.2 5.99.1.2] </span></td></tr> | ||
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6cq2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cq2 OCA], [http://pdbe.org/6cq2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6cq2 RCSB], [http://www.ebi.ac.uk/pdbsum/6cq2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6cq2 ProSAT]</span></td></tr> | ||
| + | </table> | ||
| + | == Function == | ||
| + | [[http://www.uniprot.org/uniprot/TOP1_MYCTU TOP1_MYCTU]] Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone.[HAMAP-Rule:MF_00952]<ref>PMID:20724443</ref> The C-terminus (residues 622-934) inhibits RNA cleavage by MazF4.<ref>PMID:20724443</ref> | ||
| + | == References == | ||
| + | <references/> | ||
| + | __TOC__ | ||
| + | </StructureSection> | ||
| + | [[Category: DNA topoisomerase]] | ||
| + | [[Category: Cao, N]] | ||
[[Category: Tan, K]] | [[Category: Tan, K]] | ||
[[Category: Thirunavukkaraus, A]] | [[Category: Thirunavukkaraus, A]] | ||
| - | [[Category: Tse-Dinh, Y | + | [[Category: Tse-Dinh, Y C]] |
| - | [[Category: | + | [[Category: Co-crystallization]] |
| + | [[Category: Complex with oligonucleotide and magnesium]] | ||
| + | [[Category: Isomerase]] | ||
| + | [[Category: Isomerase-dna complex]] | ||
| + | [[Category: Mycobacterium tuberculosis]] | ||
| + | [[Category: Topoisomerase i]] | ||
Revision as of 05:35, 30 May 2018
Crystal structure of Mycobacterium tuberculosis Topoisomerase I in complex with oligonucleotide MTS2-12 and Magnesium
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