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5y2e

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Revision as of 05:50, 30 May 2018

Crystal structure of the oligomerization domain of NSP4 from the rotavirus strain NCDV

Structural highlights

5y2e is a 4 chain structure with sequence from Bovine rotavirus (serotype 6 / strain ncdv). Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[NSP4_ROTBN] Involved in virus morphogenesis. Functions as a receptor for the immature double-layered inner capsid particle (ICP) which transiently buds into the lumen of the rough endoplasmic reticulum during viral maturation. Enterotoxin that causes a phospholipase C-dependent elevation of the intracellular calcium concentration in host intestinal mucosa cells. Increased concentration of intracellular calcium disrupts the cytoskeleton and the tight junctions, raising the paracellular permeability. Potentiates chloride ion secretion through a calcium ion-dependent signaling pathway, inducing age-dependent diarrhea. To perform this enterotoxigenic role in vivo, NSP4 is probably released from infected enterocytes in a soluble form capable of diffusing within the intestinal lumen and interacting with the plasma membrane receptors on neighboring epithelial cells. Possible receptors for NSP4 are alpha-1/beta-1 and alpha-2/beta-1 integrin heterodimers (By similarity).

Publication Abstract from PubMed

Rotavirus nonstructural protein 4, the first viral enterotoxin to be identified, is a multidomain, multifunctional glycoprotein. Earlier, we reported a Ca(2+)-bound coiled-coil tetrameric structure of the diarrhea-inducing region of NSP4 from the rotavirus strains SA11 and I321 and a Ca(2+)-free pentameric structure from the rotavirus strain ST3, all with a parallel arrangement of alpha-helices. pH was found to determine the oligomeric state: a basic pH favoured a tetramer, whereas an acidic pH favoured a pentamer. Here, we report two novel forms of the coiled-coil region of NSP4 from the bovine rotavirus strains MF66 and NCDV. These crystallized at acidic pH, forming antiparallel coiled-coil tetrameric structures without any bound Ca(2+) ion. Structural and mutational studies of the coiled-coil regions of NSP4 revealed that the nature of the residue at position 131 (Tyr/His) plays an important role in the observed structural diversity.

New tetrameric forms of the rotavirus NSP4 with antiparallel helices.,Kumar S, Ramappa R, Pamidimukkala K, Rao CD, Suguna K Arch Virol. 2018 Feb 17. pii: 10.1007/s00705-018-3753-6. doi:, 10.1007/s00705-018-3753-6. PMID:29455326^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Kumar S, Ramappa R, Pamidimukkala K, Rao CD, Suguna K. New tetrameric forms of the rotavirus NSP4 with antiparallel helices. Arch Virol. 2018 Feb 17. pii: 10.1007/s00705-018-3753-6. doi:, 10.1007/s00705-018-3753-6. PMID:29455326 doi:http://dx.doi.org/10.1007/s00705-018-3753-6

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5y2e"

Categories: Kumar, S | Suguna, K | Antiparallel tetramer nsp4 rotavirus coiled-coil | Viral protein

@@ Line 3: / Line 3: @@
 <StructureSection load='5y2e' size='340' side='right' caption='[[5y2e]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5y2e]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y2E FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5y2e]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bovine_rotavirus_(serotype_6_/_strain_ncdv) Bovine rotavirus (serotype 6 / strain ncdv)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y2E OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Y2E FirstGlance]. <br>
 </td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5y2e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y2e OCA], [http://pdbe.org/5y2e PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5y2e RCSB], [http://www.ebi.ac.uk/pdbsum/5y2e PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5y2e ProSAT]</span></td></tr>
 </table>

5y2e

From Proteopedia

Revision as of 05:50, 30 May 2018

Crystal structure of the oligomerization domain of NSP4 from the rotavirus strain NCDV

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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