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2iw8
From Proteopedia
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|PDB= 2iw8 |SIZE=350|CAPTION= <scene name='initialview01'>2iw8</scene>, resolution 2.30Å | |PDB= 2iw8 |SIZE=350|CAPTION= <scene name='initialview01'>2iw8</scene>, resolution 2.30Å | ||
|SITE= <scene name='pdbsite=AC1:4sp+Binding+Site+For+Chain+C'>AC1</scene> | |SITE= <scene name='pdbsite=AC1:4sp+Binding+Site+For+Chain+C'>AC1</scene> | ||
| - | |LIGAND= <scene name='pdbligand=4SP:O6-CYCLOHEXYLMETHOXY-2-(4'-SULPHAMOYLANILINO)+PURINE'>4SP</scene> | + | |LIGAND= <scene name='pdbligand=4SP:O6-CYCLOHEXYLMETHOXY-2-(4'-SULPHAMOYLANILINO)+PURINE'>4SP</scene>, <scene name='pdbligand=SGM:MONOTHIOGLYCEROL'>SGM</scene>, <scene name='pdbligand=TPO:PHOSPHOTHREONINE'>TPO</scene> |
| - | |ACTIVITY= [http://en.wikipedia.org/wiki/ | + | |ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span> |
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY= | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iw8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iw8 OCA], [http://www.ebi.ac.uk/pdbsum/2iw8 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iw8 RCSB]</span> | ||
}} | }} | ||
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Dissecting the determinants of cyclin-dependent kinase 2 and cyclin-dependent kinase 4 inhibitor selectivity., Pratt DJ, Bentley J, Jewsbury P, Boyle FT, Endicott JA, Noble ME, J Med Chem. 2006 Sep 7;49(18):5470-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16942020 16942020] | Dissecting the determinants of cyclin-dependent kinase 2 and cyclin-dependent kinase 4 inhibitor selectivity., Pratt DJ, Bentley J, Jewsbury P, Boyle FT, Endicott JA, Noble ME, J Med Chem. 2006 Sep 7;49(18):5470-7. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16942020 16942020] | ||
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
| + | [[Category: Non-specific serine/threonine protein kinase]] | ||
[[Category: Protein complex]] | [[Category: Protein complex]] | ||
| - | [[Category: Transferred entry: 2 7.11 1]] | ||
[[Category: Bentley, J.]] | [[Category: Bentley, J.]] | ||
[[Category: Boyle, F T.]] | [[Category: Boyle, F T.]] | ||
| Line 29: | Line 32: | ||
[[Category: Noble, M E.M.]] | [[Category: Noble, M E.M.]] | ||
[[Category: Pratt, D J.]] | [[Category: Pratt, D J.]] | ||
| - | [[Category: 4SP]] | ||
| - | [[Category: SGM]] | ||
[[Category: atp-binding]] | [[Category: atp-binding]] | ||
[[Category: cell cycle]] | [[Category: cell cycle]] | ||
| Line 45: | Line 46: | ||
[[Category: transferase]] | [[Category: transferase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:49:30 2008'' |
Revision as of 00:49, 31 March 2008
| |||||||
| , resolution 2.30Å | |||||||
|---|---|---|---|---|---|---|---|
| Sites: | |||||||
| Ligands: | , , | ||||||
| Activity: | Non-specific serine/threonine protein kinase, with EC number 2.7.11.1 | ||||||
| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
STRUCTURE OF HUMAN THR160-PHOSPHO CDK2-CYCLIN A F82H-L83V-H84D MUTANT WITH AN O6-CYCLOHEXYLMETHYLGUANINE INHIBITOR
Overview
Cyclin dependent kinases are a key family of kinases involved in cell cycle regulation and are an attractive target for cancer chemotherapy. The roles of four residues of the cyclin-dependent kinase active site in inhibitor selectivity were investigated by producing cyclin-dependent kinase 2 mutants bearing equivalent cyclin-dependent kinase 4 residues, namely F82H, L83V, H84D, and K89T. Assay of the mutants with a cyclin-dependent kinase 4-selective bisanilinopyrimidine shows that the K89T mutation is primarily responsible for the selectivity of this compound. Use of the cyclin-dependent kinase 2-selective 6-cyclohexylmethoxy-2-(4'-sulfamoylanilino)purine (NU6102) shows that K89T has no role in the selectivity, while the remaining three mutations have a cumulative influence. The results indicate that certain residues that are not frequently considered in structure-aided kinase inhibitor design have an important role to play.
About this Structure
2IW8 is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Dissecting the determinants of cyclin-dependent kinase 2 and cyclin-dependent kinase 4 inhibitor selectivity., Pratt DJ, Bentley J, Jewsbury P, Boyle FT, Endicott JA, Noble ME, J Med Chem. 2006 Sep 7;49(18):5470-7. PMID:16942020
Page seeded by OCA on Mon Mar 31 03:49:30 2008
Categories: Homo sapiens | Non-specific serine/threonine protein kinase | Protein complex | Bentley, J. | Boyle, F T. | Endicott, J A. | Jewsbury, P. | Noble, M E.M. | Pratt, D J. | Atp-binding | Cell cycle | Cell cycle complex | Cell division | Cyclin | Kinase | Mitosis | Nucleotide-binding | Phosphorylation | Polymorphism | Serine-threonine-protein kinase | Serine/threonine-protein kinase | Transferase
