User:Ricardo Alberto Chiong Zevallos/Sandbox 1
From Proteopedia
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== Function == | == Function == | ||
| - | BMI1 is a ring finger protein that is component of a <scene name='78/787701/2h0d/1'>Polycomb group (PcG) multiprotein PRC1 complex</scene>, which epigenetically repress regulatory genes, such as Hox genes, related to embryonic development and self-renewal of somatic stem-cells. BMI1 is central in the DNA damage repair, being the aberrant expression associated with numerous cancers and resistance to some types of chemotherapies. | + | B-cell-specific Moloney leukemia virus insertion site 1 (BMI1) is a ring finger protein that is component of a <scene name='78/787701/2h0d/1'>Polycomb group (PcG) multiprotein PRC1 complex</scene>, which epigenetically repress regulatory genes, such as Hox genes, related to embryonic development and self-renewal of somatic stem-cells. BMI1 is central in the DNA damage repair, being the aberrant expression associated with numerous cancers and resistance to some types of chemotherapies. |
PcG PRC1 is responsible for chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. BMI1 regulates the E3 ubiquitin-protein ligase activity of RING1b, which is also a protein component of the PcG PRC1 complex. Other monomers such as CBX2, CBX4, CBX8, PHC1, PHC2 and RNF2/RING2 are capable of forming a PRC1-like complex replacing the RING1b, also forming a heterodimer with E3 ubiquitin activity. In the complex, <scene name='78/787701/Bmi1_destacada_de_2h0d/1'>BMI1</scene> and <scene name='78/787701/Ring1b_destacada_de_2h0d/1'>RING1b</scene> form a heterodimer and only RING1b interacts with UbcH5c, which is a Ubiquitin Conjugating Enzyme E2. | PcG PRC1 is responsible for chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. BMI1 regulates the E3 ubiquitin-protein ligase activity of RING1b, which is also a protein component of the PcG PRC1 complex. Other monomers such as CBX2, CBX4, CBX8, PHC1, PHC2 and RNF2/RING2 are capable of forming a PRC1-like complex replacing the RING1b, also forming a heterodimer with E3 ubiquitin activity. In the complex, <scene name='78/787701/Bmi1_destacada_de_2h0d/1'>BMI1</scene> and <scene name='78/787701/Ring1b_destacada_de_2h0d/1'>RING1b</scene> form a heterodimer and only RING1b interacts with UbcH5c, which is a Ubiquitin Conjugating Enzyme E2. | ||
Revision as of 14:29, 10 June 2018
Contents |
BMI1
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You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue.
Urgente: nesse link eu coloquei os artigos para procurar os detalhes estruturais, ainda não entendi como pegar a estrutura isolada da BMI1 (sem as outras que formam o complexo PRC1) e mostrar no pymol: https://drive.google.com/drive/folders/1l195aNuY6joOd74GKKxa-XWTRMBv_uWF?usp=sharing pode apagar esse recado depois que você ler, vou deixar o link nas referências
Function
B-cell-specific Moloney leukemia virus insertion site 1 (BMI1) is a ring finger protein that is component of a , which epigenetically repress regulatory genes, such as Hox genes, related to embryonic development and self-renewal of somatic stem-cells. BMI1 is central in the DNA damage repair, being the aberrant expression associated with numerous cancers and resistance to some types of chemotherapies.
PcG PRC1 is responsible for chromatin remodeling and histone modification, mediating monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. BMI1 regulates the E3 ubiquitin-protein ligase activity of RING1b, which is also a protein component of the PcG PRC1 complex. Other monomers such as CBX2, CBX4, CBX8, PHC1, PHC2 and RNF2/RING2 are capable of forming a PRC1-like complex replacing the RING1b, also forming a heterodimer with E3 ubiquitin activity. In the complex, and form a heterodimer and only RING1b interacts with UbcH5c, which is a Ubiquitin Conjugating Enzyme E2.
Disease
Relevance
Structure
In the PRC1 complex, Bmi1 and Ring1b heterodimerize via their N-terminal RING domains, forming an active E3 ubiquitin ligase. A RING-domain E3 is responsible for promoting the transfer of ubiquitin from the active site of the E2 ubiquitin-conjugating enzyme to an acceptor lysine residue in the substrate. The PRC1 complex promote the monoubiquitination of histone H2A on lysine 119 (uH2A), which leads to the stalling of the RNA polymerase at the promotor of the monoubiquitinated gene, hence the transcription doesn't occur. HoxC13 gene is one of the Hox gene regulated by the PRC1 complex during development.
How can the PRC1 complex promote such a epigenetic modification? The complex binds to the duplex of DNA through a surface patch unique to the Bmi1/Ring1b RING–RING dimer. However only the RING1b interacts with the E2 enzyme UbcH5c. The ubiquitination is a cascade of events that involve a E1 activating enzyme, a E2 ubiquitin-conjugating enzyme and a E3 ubiquitin-protein ligase. First, E1 receives the ubiquitin through the covvalent attaching of the ubiquitin C terminus to a cysteine of the E1 through a thioester bond, only possible due to ATP hydrolysis. Then, the E1 ubiquitin conjugate binds to an E2, catalyzing the transfer of the ubiquitin onto the E2 active site cysteine, also by a thio ester bond. Finally, the E3 ligase brings together the loaded E2 and the substrate, catalyzing the transfer of ubiquitin from the active site cysteine of the E2 to the amino group of a lysine side chain forming a covalent bond. Therefore, the PRC1 complex promotes the transfer of the ubiquitin attached to E2 enzyme UbcH5c to the lysine 119 on histone H2A.
</StructureSection>
References
- ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
- ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
[| BMI1 in NCBI] [| BMI1 in NCBI] [| BMI1 in NCBI] https://www.genecards.org/cgi-bin/carddisp.pl?gene=UBE2D3 https://www.genenames.org/cgi-bin/genefamilies/set/58 https://www.cellsignal.com/contents/resources-protein-domains-interactions/ring-protein-domain/domains-ring http://www.ebi.ac.uk/interpro/entry/IPR001841
https://drive.google.com/drive/folders/1l195aNuY6joOd74GKKxa-XWTRMBv_uWF?usp=sharing
