2j53
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= | |GENE= | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1em7|1EM7]], [[1gb1|1GB1]], [[1igc|1IGC]], [[1igd|1IGD]], [[1le3|1LE3]], [[1mpe|1MPE]], [[1mvk|1MVK]], [[1pga|1PGA]], [[1pgb|1PGB]], [[1pgx|1PGX]], [[1pn5|1PN5]], [[1q10|1Q10]], [[2gb1|2GB1]], [[2igd|2IGD]], [[2igh|2IGH]], [[2j52|2J52]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2j53 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2j53 OCA], [http://www.ebi.ac.uk/pdbsum/2j53 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2j53 RCSB]</span> | ||
}} | }} | ||
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[[Category: protein g]] | [[Category: protein g]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:53:01 2008'' |
Revision as of 00:53, 31 March 2008
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| Related: | 1EM7, 1GB1, 1IGC, 1IGD, 1LE3, 1MPE, 1MVK, 1PGA, 1PGB, 1PGX, 1PN5, 1Q10, 2GB1, 2IGD, 2IGH, 2J52
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
SOLUTION STRUCTURE OF GB1 DOMAIN PROTEIN G AND LOW AND HIGH PRESSURE.
Overview
The solution structure of the GB1 domain of protein G at a pressure of 2 kbar is presented. The structure was calculated as a change from an energy-minimised low-pressure structure using (1)H chemical shifts. Two separate changes can be characterised: a compression/distortion, which is linear with pressure; and a stabilisation of an alternative folded state. On application of pressure, linear chemical shift changes reveal that the backbone structure changes by about 0.2 A root mean square, and is compressed by about 1% overall. The alpha-helix compresses, particularly at the C-terminal end, and moves toward the beta-sheet, while the beta-sheet is twisted, with the corners closest to the alpha-helix curling up towards it. The largest changes in structure are along the second beta-strand, which becomes more twisted. This strand is where the protein binds to IgG. Curved chemical shift changes with pressure indicate that high pressure also populates an alternative structure with a distortion towards the C-terminal end of the helix, which is likely to be caused by insertion of a water molecule. Proteins 2007. (c) 2007 Wiley-Liss, Inc.
About this Structure
2J53 is a Single protein structure of sequence from Streptococcus sp.. Full crystallographic information is available from OCA.
Reference
Pressure-induced changes in the solution structure of the GB1 domain of protein G., Wilton DJ, Tunnicliffe RB, Kamatari YO, Akasaka K, Williamson MP, Proteins. 2007 Dec 12;. PMID:18076052
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