5yke

From Proteopedia

(Difference between revisions)

Revision as of 08:07, 14 June 2018

Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (focused refinement on TM at 4.11A)

Structural highlights

5yke is a 8 chain structure with sequence from Golden hamster and Lk3 transgenic mice. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	Kcnj11 (LK3 transgenic mice), Abcc8 (Golden hamster)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[KCJ11_MOUSE] This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium. Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation (By similarity).

Publication Abstract from PubMed

ATP-sensitive potassium channels (KATP) are energy sensors on the plasma membrane. By sensing the intracellular ADP/ATP ratio of beta-cells, pancreatic KATP channels control insulin release and regulate metabolism at the whole body level. They are implicated in many metabolic disorders and diseases and are therefore important drug targets. Here, we present three structures of pancreatic KATP channels solved by cryo-electron microscopy (cryo-EM), at resolutions ranging from 4.1 to 4.5 A. These structures depict the binding site of the antidiabetic drug glibenclamide, indicate how Kir6.2 (inward-rectifying potassium channel 6.2) N-terminus participates in the coupling between the peripheral SUR1 (sulfonylurea receptor 1) subunit and the central Kir6.2 channel, reveal the binding mode of activating nucleotides, and suggest the mechanism of how Mg-ADP binding on nucleotide binding domains (NBDs) drives a conformational change of the SUR1 subunit.

Ligand binding and conformational changes of SUR1 subunit in pancreatic ATP-sensitive potassium channels.,Wu JX, Ding D, Wang M, Kang Y, Zeng X, Chen L Protein Cell. 2018 Mar 28. pii: 10.1007/s13238-018-0530-y. doi:, 10.1007/s13238-018-0530-y. PMID:29594720^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wu JX, Ding D, Wang M, Kang Y, Zeng X, Chen L. Ligand binding and conformational changes of SUR1 subunit in pancreatic ATP-sensitive potassium channels. Protein Cell. 2018 Mar 28. pii: 10.1007/s13238-018-0530-y. doi:, 10.1007/s13238-018-0530-y. PMID:29594720 doi:http://dx.doi.org/10.1007/s13238-018-0530-y

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5yke"

@@ Line 3: / Line 3: @@
 <StructureSection load='5yke' size='340' side='right' caption='[[5yke]], [[Resolution|resolution]] 4.11&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5yke]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YKE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YKE FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5yke]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/Golden_hamster Golden hamster] and [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YKE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YKE FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GBM:5-CHLORO-N-(2-{4-[(CYCLOHEXYLCARBAMOYL)SULFAMOYL]PHENYL}ETHYL)-2-METHOXYBENZAMIDE'>GBM</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Kcnj11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice]), Abcc8 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10036 Golden hamster])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yke FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yke OCA], [http://pdbe.org/5yke PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yke RCSB], [http://www.ebi.ac.uk/pdbsum/5yke PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yke ProSAT]</span></td></tr>
 </table>
@@ Line 22: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Golden hamster]]
+[[Category: Lk3 transgenic mice]]
 [[Category: Chen, L]]
 [[Category: Wu, J X]]

5yke

From Proteopedia

Revision as of 08:07, 14 June 2018

Structure of pancreatic ATP-sensitive potassium channel bound with glibenclamide and ATPgammaS (focused refinement on TM at 4.11A)

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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