This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

2eqt

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 08:28, 14 June 2018

Micelle-bound structure of growth-blocking peptide of the armyworm, Pseudaletia separata

Structural highlights

2eqt is a 1 chain structure with sequence from Leucania separata. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Related:	2eqh, 2eqq
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[GBP_MYTSE] Biogenic peptide that prevents the onset of metamorphosis from larva to pupa. Has repressive activity against juvenile hormone esterase. Induces cell proliferation at low concentrations and inhibits it at high concentrations. Mediates the spreading of plasmatocytes to foreign surfaces. May have a role in the regulation of dopamine concentrations.^[1] ^[2] ^[3]

Publication Abstract from PubMed

Growth-blocking peptide (GBP) is a hormone-like peptide that suppresses the growth of the host armyworm. Although the 23-amino acid GBP (1-23 GBP) is expressed in nonparasitized armyworm plasma, the parasitization by wasp produces the 28-amino acid GBP (1-28 GBP) through an elongation of the C-terminal amino acid sequence. In this study, we characterized the GBP variants, which consist of various lengths of the C-terminal region, by comparing their biological activities and three-dimensional structures. The results of an injection study indicate that 1-28 GBP most strongly suppresses larval growth. NMR analysis shows that these peptides have basically the same tertiary structures and that the extension of the C-terminal region is disordered. However, the C-terminal region of 1-28 GBP undergoes a conformational transition from a random coiled state to an alpha-helical state in the presence of dodecylphosphocholine micelles. This suggests that binding of the C-terminal region would affect larval growth activity.

C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity.,Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K J Biol Chem. 2009 Oct 23;284(43):29625-34. Epub 2009 Aug 26. PMID:19710009^[4]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Hayakawa Y, Ohnishi A, Mizoguchi A, Yamashika C. Distribution of growth-blocking peptide in the insect central nervous tissue. Cell Tissue Res. 2000 Jun;300(3):459-64. PMID:10928276
↑ Aizawa T, Hayakawa Y, Ohnishi A, Fujitani N, Clark KD, Strand MR, Miura K, Koganesawa N, Kumaki Y, Demura M, Nitta K, Kawano K. Structure and activity of the insect cytokine growth-blocking peptide. Essential regions for mitogenic and hemocyte-stimulating activities are separate. J Biol Chem. 2001 Aug 24;276(34):31813-8. Epub 2001 Jun 27. PMID:11429413 doi:http://dx.doi.org/10.1074/jbc.M105251200
↑ Hayakawa Y, Ohnishi A. Cell growth activity of growth-blocking peptide. Biochem Biophys Res Commun. 1998 Sep 18;250(2):194-9. PMID:9753606 doi:http://dx.doi.org/10.1006/bbrc.1998.8959
↑ Umetsu Y, Aizawa T, Muto K, Yamamoto H, Kamiya M, Kumaki Y, Mizuguchi M, Demura M, Hayakawa Y, Kawano K. C-terminal elongation of growth-blocking peptide enhances its biological activity and micelle binding affinity. J Biol Chem. 2009 Oct 23;284(43):29625-34. Epub 2009 Aug 26. PMID:19710009 doi:10.1074/jbc.M109.011148

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2eqt"

@@ Line 1: / Line 1: @@
 ==Micelle-bound structure of growth-blocking peptide of the armyworm, Pseudaletia separata==
 <StructureSection load='2eqt' size='340' side='right' caption='[[2eqt]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''>
@@ Line 4: / Line 5: @@
 <table><tr><td colspan='2'>[[2eqt]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Leucania_separata Leucania separata]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EQT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2EQT FirstGlance]. <br>
 </td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[2eqh|2eqh]], [[2eqq|2eqq]]</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eqt OCA], [http://pdbe.org/2eqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eqt RCSB], [http://www.ebi.ac.uk/pdbsum/2eqt PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2eqt FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2eqt OCA], [http://pdbe.org/2eqt PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2eqt RCSB], [http://www.ebi.ac.uk/pdbsum/2eqt PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2eqt ProSAT]</span></td></tr>
 </table>
 == Function ==

2eqt

From Proteopedia

Revision as of 08:28, 14 June 2018

Micelle-bound structure of growth-blocking peptide of the armyworm, Pseudaletia separata

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox