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2fok

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Revision as of 08:50, 14 June 2018

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI

Structural highlights

2fok is a 2 chain structure with sequence from Atcc 33414. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	FOKI (ATCC 33414)
Activity:	Type II site-specific deoxyribonuclease, with EC number 3.1.21.4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[T2F1_PLAOK] Recognizes the double-stranded sequence 5'-GGATG-3'/3'-CATCC-5' and cleaves respectively 14 bases after G-1 and 13 bases before C-1.

Publication Abstract from PubMed

FokI is a member an unusual class of restriction enzymes that recognize a specific DNA sequence and cleave nonspecifically a short distance away from that sequence. FokI consists of an N-terminal DNA recognition domain and a C-terminal cleavage domain. The bipartite nature of FokI has led to the development of artificial enzymes with novel specificities. We have solved the structure of FokI to 2.3 A resolution. The structure reveals a dimer, in which the dimerization interface is mediated by the cleavage domain. Each monomer has an overall conformation similar to that found in the FokI-DNA complex, with the cleavage domain packing alongside the DNA recognition domain. In corroboration with the cleavage data presented in the accompanying paper in this issue of Proceedings, we propose a model for FokI DNA cleavage that requires the dimerization of FokI on DNA to cleave both DNA strands.

Structure of FokI has implications for DNA cleavage.,Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. PMID:9724743^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Wah DA, Bitinaite J, Schildkraut I, Aggarwal AK. Structure of FokI has implications for DNA cleavage. Proc Natl Acad Sci U S A. 1998 Sep 1;95(18):10564-9. PMID:9724743

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2fok"

@@ Line 1: / Line 1: @@
 ==STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI==
 <StructureSection load='2fok' size='340' side='right' caption='[[2fok]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
@@ Line 5: / Line 6: @@
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">FOKI ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=244 ATCC 33414])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fok OCA], [http://pdbe.org/2fok PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fok RCSB], [http://www.ebi.ac.uk/pdbsum/2fok PDBsum]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2fok FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2fok OCA], [http://pdbe.org/2fok PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2fok RCSB], [http://www.ebi.ac.uk/pdbsum/2fok PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2fok ProSAT]</span></td></tr>
 </table>
 == Function ==

2fok

From Proteopedia

Revision as of 08:50, 14 June 2018

STRUCTURE OF RESTRICTION ENDONUCLEASE FOKI

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

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