2jmc
From Proteopedia
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|ACTIVITY= | |ACTIVITY= | ||
|GENE= SPTAN1, SPTA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | |GENE= SPTAN1, SPTA2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9031 Gallus gallus]) | ||
| + | |DOMAIN= | ||
| + | |RELATEDENTRY=[[1tuc|1TUC]], [[2jma|2JMA]] | ||
| + | |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2jmc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2jmc OCA], [http://www.ebi.ac.uk/pdbsum/2jmc PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2jmc RCSB]</span> | ||
}} | }} | ||
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[[Category: spc-sh3]] | [[Category: spc-sh3]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:59:10 2008'' |
Revision as of 00:59, 31 March 2008
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| Gene: | SPTAN1, SPTA2 (Gallus gallus) | ||||||
| Related: | 1TUC, 2JMA
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| Resources: | FirstGlance, OCA, PDBsum, RCSB | ||||||
| Coordinates: | save as pdb, mmCIF, xml | ||||||
Chimer between Spc-SH3 and P41
Overview
Here we present the high-resolution NMR structure of a chimera (SPCp41) between alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics perfectly the interactions typically found in SH3-peptide complexes and is remarkably similar to that of the complex between the separate Spc-SH3 domain and ligand p41. Relaxation data confirm the tight binding between the ligand and SH3 part of the chimera. This chimera will serve as a tool for a deeper understanding of the relationship between structure and thermodynamics of binding using a combination of NMR, stability and site-directed mutagenesis studies, which can lead to an effective strategy for ligand design.
About this Structure
2JMC is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex., Candel AM, Conejero-Lara F, Martinez JC, van Nuland NA, Bruix M, FEBS Lett. 2007 Feb 20;581(4):687-92. Epub 2007 Jan 22. PMID:17275816
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